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• W1981257647 abstract "This study presents a method for dissociating parotid gland tissue into separate acinar groups, single acini, cell clumps and single cells for the in vitro study of secretion. Parotid slices, in Saline G plus special additives, were treated with 5 mg/ml crude collagenase and 1 mg/ ml soybean trypsin inhibitor followed by mechanical agitation and rinsing in fresh medium plus BSA. The resulting suspension was stimulated with 10 μM isoprenaline and compared with nondissociated parotid slices for the release of amylase over a 90 min period. About 12 per cent of the amylase of dissociated tissue was present in the medium at time zero, as compared with 0.5 per cent of the amylase of sliced tissue but after 90 min stimulation both preparations released about 76 per cent of total amylase. Specimens monitored by light and electron microscopy showed a loss of secretion granules in stimulated cells and the formation of lysosomes. Scanning electron microscopy showed that stromal elements disappeared after collagenase treatment and that the basal lamina was absent in finely divided cells which also demonstrated surface blebs, microvilli and ruffles." @default.
• W1981257647 created "2016-06-24" @default.
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• W1981257647 date "1975-03-01" @default.
• W1981257647 modified "2023-09-25" @default.
• W1981257647 title "Morphologic and biochemical study of dissociated rat parotid gland acini stimulated in vitro" @default.
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• W1981257647 doi "https://doi.org/10.1016/0003-9969(75)90001-1" @default.
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