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- W1981293044 abstract "A single-step, highly specific and easy-to-use method was developed for isolation and purification of melatonin from complex biological matrices. Polyclonal antibodies highly specific against melatonin (with cross-reactivities with related compounds below 0.02%, except for 6-hydroxymelatonin) were raised, characterised by enzyme-linked immunosorbent assay (ELISA) and used for preparation of immunoaffinity gel. Melatonin recovery by the immunoaffinity method was approximately 95%, allowing single-step processing of samples prior to electrospray HPLC-MS analysis (with detection limit 10 fmol). The method was successfully used for determining melatonin in human serum and turned out to be better than the non-specific solid-phase extraction published earlier." @default.
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- W1981293044 date "2002-07-01" @default.
- W1981293044 modified "2023-10-18" @default.
- W1981293044 title "Isolation of melatonin by immunoaffinity chromatography" @default.
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- W1981293044 doi "https://doi.org/10.1016/s1570-0232(02)00246-5" @default.
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