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• W1981295117 abstract "The principle of a novel latex agglutination test based on combined results of electrophoretic mobility and particle aggregation measurements is described. Poly(styrene/alpha-tert-butoxy-omega-vinylbenzylpolyglycidol) (P(S/PGL)) microspheres were synthesized by a one step soap-free emulsion copolymerization of styrene and alpha-tert-butoxy-omega-vinylbenzylpolyglycidol macromonomer with number average molecular weight Mn = 2700 (polydispersity [Mw]/[Mn] = 1.10). Particles with monomodal size distribution (number average diameter Dn = 220 nm) and surface fraction of polyglycidol equal to f = 0.42 mol % were obtained. Human serum albumin (HSA) was covalently bound onto the surface of P(S/PGL) microspheres activated with 1,3,5-trichlorotriazine. In a model immunodiagnostic assay for anti-HSA, in which P(S/PGL) particles with covalently bound HSA have been used, the electrophoretic mobility and aggregation of microspheres were measured simultaneously. This approach allowed detection of anti-HSA in the serum in the range of anti-HSA concentrations from 0.1 to 150 microg/mL. The highest changes in electrophoretic mobility were registered for microspheres with surface concentration of immobilized HSA equal to Gamma = 9.2 x 10(-4) g/m2." @default.
• W1981295117 created "2016-06-24" @default.
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• W1981295117 date "2003-09-10" @default.
• W1981295117 modified "2023-10-11" @default.
• W1981295117 title "Poly(styrene/α-<i>tert</i>-butoxy-ω-vinylbenzylpolyglycidol) Microspheres for Immunodiagnostics. Principle of a Novel Latex Test Based on Combined Electrophoretic Mobility and Particle Aggregation Measurements" @default.
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• W1981295117 doi "https://doi.org/10.1021/bm0342887" @default.
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