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- W1981332009 abstract "Histopathological diagnosis of bacterial infection remains a technical challenge. Application of histochemistry provides a prospect of the improvement of diagnostic quality. Methicillin-resistant Staphylococcus aureus (MRSA), the most common drug-resistant bacterium, is of clinical importance in making appropriate histopathological diagnosis. Recently, community-acquired MRSA has expanded, in addition to conventional hospital-acquired MRSA. Immunohistochemical identification of MRSA requires antibodies against both species-specific antigens and penicillin-binding protein 2′ (PBP2′), whereas a species-specific gene and mec A encoding PBP2′ can be the target of in situ hybridization (ISH) detection. Specificity verification in histochemistry should be emphasized, since S. aureus commonly expresses protein A in the cell wall, whose immunoglobulin-binding capacity is retrieved by heating pretreatment of routinely prepared sections. The ISH technique for MRSA needs meticulous pretreatment of routine paraffin-embedded sections and signal enhancement sequence. This review focuses on such histopathological approaches, which should have profound potential for contributing to decreasing and preventing nosocomial infection of MRSA." @default.
- W1981332009 created "2016-06-24" @default.
- W1981332009 creator A5071766101 @default.
- W1981332009 creator A5076676217 @default.
- W1981332009 date "2007-11-01" @default.
- W1981332009 modified "2023-09-26" @default.
- W1981332009 title "Histochemical identification of Methicillin-resistant Staphylococcus aureus: contribution to preventing nosocomial infection" @default.
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- W1981332009 doi "https://doi.org/10.1053/j.semdp.2007.07.004" @default.
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