Matches in SemOpenAlex for { <https://semopenalex.org/work/W1981342015> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W1981342015 endingPage "282" @default.
- W1981342015 startingPage "277" @default.
- W1981342015 abstract "The structural gene for a subtilisin J from Bacillus stearothermophilus NCIMB10278 was cloned in Bacillus subtilis using pZ124 as a vector, and its nucleotide sequence was determined. The nucleotide sequence revealed only one large open reading frame, composed of 1,143 base pairs and 381 amino acid residues. A Shine-Dalgarno sequence was found 8 bp upstream from the translation start site (GTG). The deduced amino acid sequence revealed an N-terminal signal peptide and pro-peptide of 106 residues followed by the mature protein comprised of 275 residues. The productivity of subtilisin in the culture broth of the Bacillus subtilis was about 46-fold higher than that of the Bacillus stearothermophilus. The amino acid sequence of the extracellular alkaline protease subtilisin J is highly homologous to that of subtilisin E and it shows 69% identity with subtilisin Carlsberg, 89% with subtilisin BPN' and 70% with subtilisin DY. Some properties of the subtilisin J that had been purified from the Bacillus subtilis were examined. The subtilisin J has alkaline pH characteristics and a molecular weight of 27,500. It retains about 50% of its activity even after treatment at 60 degrees C for 30 min in the presence of 2 mM calcium chloride." @default.
- W1981342015 created "2016-06-24" @default.
- W1981342015 creator A5007606574 @default.
- W1981342015 creator A5052571056 @default.
- W1981342015 creator A5061102292 @default.
- W1981342015 creator A5067366069 @default.
- W1981342015 date "1992-04-01" @default.
- W1981342015 modified "2023-10-16" @default.
- W1981342015 title "Molecular cloning of a subtilisin J gene from Bacillus stearothermophilus and its expression in Bacillus subtilis" @default.
- W1981342015 cites W1566971930 @default.
- W1981342015 cites W1568173496 @default.
- W1981342015 cites W1776082650 @default.
- W1981342015 cites W1802247160 @default.
- W1981342015 cites W1806837705 @default.
- W1981342015 cites W1816537780 @default.
- W1981342015 cites W1945765509 @default.
- W1981342015 cites W1965018976 @default.
- W1981342015 cites W1968416749 @default.
- W1981342015 cites W1979263745 @default.
- W1981342015 cites W2015204736 @default.
- W1981342015 cites W2034756360 @default.
- W1981342015 cites W2067133968 @default.
- W1981342015 cites W2074904307 @default.
- W1981342015 cites W2091198733 @default.
- W1981342015 cites W2138913088 @default.
- W1981342015 cites W2156311145 @default.
- W1981342015 cites W2170119500 @default.
- W1981342015 cites W2170501882 @default.
- W1981342015 doi "https://doi.org/10.1016/0006-291x(92)91189-w" @default.
- W1981342015 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1567435" @default.
- W1981342015 hasPublicationYear "1992" @default.
- W1981342015 type Work @default.
- W1981342015 sameAs 1981342015 @default.
- W1981342015 citedByCount "38" @default.
- W1981342015 countsByYear W19813420152012 @default.
- W1981342015 countsByYear W19813420152013 @default.
- W1981342015 countsByYear W19813420152014 @default.
- W1981342015 countsByYear W19813420152015 @default.
- W1981342015 countsByYear W19813420152017 @default.
- W1981342015 countsByYear W19813420152019 @default.
- W1981342015 crossrefType "journal-article" @default.
- W1981342015 hasAuthorship W1981342015A5007606574 @default.
- W1981342015 hasAuthorship W1981342015A5052571056 @default.
- W1981342015 hasAuthorship W1981342015A5061102292 @default.
- W1981342015 hasAuthorship W1981342015A5067366069 @default.
- W1981342015 hasConcept C100544194 @default.
- W1981342015 hasConcept C104317684 @default.
- W1981342015 hasConcept C10858879 @default.
- W1981342015 hasConcept C153911025 @default.
- W1981342015 hasConcept C167625842 @default.
- W1981342015 hasConcept C181199279 @default.
- W1981342015 hasConcept C2777272437 @default.
- W1981342015 hasConcept C2777811704 @default.
- W1981342015 hasConcept C2779811187 @default.
- W1981342015 hasConcept C523546767 @default.
- W1981342015 hasConcept C54355233 @default.
- W1981342015 hasConcept C55493867 @default.
- W1981342015 hasConcept C84148353 @default.
- W1981342015 hasConcept C86803240 @default.
- W1981342015 hasConceptScore W1981342015C100544194 @default.
- W1981342015 hasConceptScore W1981342015C104317684 @default.
- W1981342015 hasConceptScore W1981342015C10858879 @default.
- W1981342015 hasConceptScore W1981342015C153911025 @default.
- W1981342015 hasConceptScore W1981342015C167625842 @default.
- W1981342015 hasConceptScore W1981342015C181199279 @default.
- W1981342015 hasConceptScore W1981342015C2777272437 @default.
- W1981342015 hasConceptScore W1981342015C2777811704 @default.
- W1981342015 hasConceptScore W1981342015C2779811187 @default.
- W1981342015 hasConceptScore W1981342015C523546767 @default.
- W1981342015 hasConceptScore W1981342015C54355233 @default.
- W1981342015 hasConceptScore W1981342015C55493867 @default.
- W1981342015 hasConceptScore W1981342015C84148353 @default.
- W1981342015 hasConceptScore W1981342015C86803240 @default.
- W1981342015 hasFunder F4320331163 @default.
- W1981342015 hasIssue "1" @default.
- W1981342015 hasLocation W19813420151 @default.
- W1981342015 hasLocation W19813420152 @default.
- W1981342015 hasOpenAccess W1981342015 @default.
- W1981342015 hasPrimaryLocation W19813420151 @default.
- W1981342015 hasRelatedWork W1776082650 @default.
- W1981342015 hasRelatedWork W1981342015 @default.
- W1981342015 hasRelatedWork W2013801100 @default.
- W1981342015 hasRelatedWork W2038693032 @default.
- W1981342015 hasRelatedWork W2055788654 @default.
- W1981342015 hasRelatedWork W2061397393 @default.
- W1981342015 hasRelatedWork W2092703433 @default.
- W1981342015 hasRelatedWork W2096217709 @default.
- W1981342015 hasRelatedWork W2131380304 @default.
- W1981342015 hasRelatedWork W2145094693 @default.
- W1981342015 hasVolume "184" @default.
- W1981342015 isParatext "false" @default.
- W1981342015 isRetracted "false" @default.
- W1981342015 magId "1981342015" @default.
- W1981342015 workType "article" @default.