FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1981422762> ?p ?o ?g. }

• W1981422762 endingPage "753" @default.
• W1981422762 startingPage "744" @default.
• W1981422762 abstract "Polysialyltransferases ST8SiaII/STX and ST8SiaIV/PST add polysialic acid (PSA) to the neural cell adhesion molecule (NCAM). Surface-located PSA is involved in cell-cell interactions participating in structural and functional plasticity of neuronal circuits. This study was undertaken to investigate the polysialyltransferase regulation pattern during hippocampal development. Polysialyltransferase expression levels analyzed by real-time RT-PCR indicated that ST8SiaII/STX mRNA is markedly down-regulated in vivo, decreasing abruptly at about the first week of postnatal development. ST8SiaII/STX mRNA is also down-regulated in hippocampal cells in culture, accompanying the morphological differentiation of neuronal interconnectivity. In contrast, ST8SiaIV/PST levels remain comparatively low during hippocampus ontogeny. Immunolabeling of primary hippocampal culture assays demonstrated that PSA expression parallels ST8SiaII/STX mRNA levels. In comparison, polysialyltransferase mRNA levels are not regulated in neuroblastoma cells during their proliferation. Sequence analysis of the 3'-untranslated region of ST8SiaII/STX cDNA indicated putative regulatory motifs. This information and the observed changes in mRNA half-life during development suggest that ST8SiaII/STX might be also regulated at the posttranscriptional level. To understand the reasons for the tight control of ST8SiaII/STX expression during development, we overexpressed the enzyme in hippocampal primary cultures by transfection. Overexpression of ST8SiaII/STX wild type as well as of a mutant lacking enzymatic activity affected neuronal viability, leading to cell death. However, this phenomenon was abolished by a double mutation in the ST8SiaII/STX that prevents formation of its three-dimentional structure. Interestingly, the overexpressed polysialyltransferase accumulates not only in the perinuclear region but also in the plasma membrane. Thus, overexpression of an ST8SiaII/STX that conserves its structure leads to abnormal accumulation of the protein, probably on the neuronal surface, affecting cell viability. This result explains the importance of an accurate regulation of polysialyltransferase expression during development." @default.
• W1981422762 created "2016-06-24" @default.
• W1981422762 creator A5024976971 @default.
• W1981422762 creator A5030918145 @default.
• W1981422762 creator A5081814176 @default.
• W1981422762 date "2003-11-17" @default.
• W1981422762 modified "2023-10-14" @default.
• W1981422762 title "Differential regulation of polysialyltransferase expression during hippocampus development: Implications for neuronal survival" @default.
• W1981422762 cites W1494919617 @default.
• W1981422762 cites W1498373535 @default.
• W1981422762 cites W1595801567 @default.
• W1981422762 cites W1673564132 @default.
• W1981422762 cites W1964709514 @default.
• W1981422762 cites W1965822810 @default.
• W1981422762 cites W1970811058 @default.
• W1981422762 cites W1975149550 @default.
• W1981422762 cites W1983825931 @default.
• W1981422762 cites W1985936398 @default.
• W1981422762 cites W1990604735 @default.
• W1981422762 cites W1990669582 @default.
• W1981422762 cites W1992304266 @default.
• W1981422762 cites W1998933962 @default.
• W1981422762 cites W2005169999 @default.
• W1981422762 cites W2006376420 @default.
• W1981422762 cites W2010576570 @default.
• W1981422762 cites W2016465082 @default.
• W1981422762 cites W2029587634 @default.
• W1981422762 cites W2034918699 @default.
• W1981422762 cites W2044908499 @default.
• W1981422762 cites W2058168695 @default.
• W1981422762 cites W2065459365 @default.
• W1981422762 cites W2067762612 @default.
• W1981422762 cites W2085402247 @default.
• W1981422762 cites W2085680371 @default.
• W1981422762 cites W2089116223 @default.
• W1981422762 cites W2104820327 @default.
• W1981422762 cites W2106698229 @default.
• W1981422762 cites W2118077969 @default.
• W1981422762 cites W2150705960 @default.
• W1981422762 cites W4244867186 @default.
• W1981422762 doi "https://doi.org/10.1002/jnr.10781" @default.
• W1981422762 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14635225" @default.
• W1981422762 hasPublicationYear "2003" @default.
• W1981422762 type Work @default.
• W1981422762 sameAs 1981422762 @default.
• W1981422762 citedByCount "36" @default.
• W1981422762 countsByYear W19814227622012 @default.
• W1981422762 countsByYear W19814227622014 @default.
• W1981422762 countsByYear W19814227622016 @default.
• W1981422762 countsByYear W19814227622017 @default.
• W1981422762 countsByYear W19814227622018 @default.
• W1981422762 countsByYear W19814227622021 @default.
• W1981422762 countsByYear W19814227622023 @default.
• W1981422762 crossrefType "journal-article" @default.
• W1981422762 hasAuthorship W1981422762A5024976971 @default.
• W1981422762 hasAuthorship W1981422762A5030918145 @default.
• W1981422762 hasAuthorship W1981422762A5081814176 @default.
• W1981422762 hasConcept C104317684 @default.
• W1981422762 hasConcept C105580179 @default.
• W1981422762 hasConcept C122871604 @default.
• W1981422762 hasConcept C148762608 @default.
• W1981422762 hasConcept C1491633281 @default.
• W1981422762 hasConcept C153911025 @default.
• W1981422762 hasConcept C169760540 @default.
• W1981422762 hasConcept C2778993516 @default.
• W1981422762 hasConcept C54009773 @default.
• W1981422762 hasConcept C54355233 @default.
• W1981422762 hasConcept C81885089 @default.
• W1981422762 hasConcept C85789140 @default.
• W1981422762 hasConcept C86803240 @default.
• W1981422762 hasConcept C95444343 @default.
• W1981422762 hasConceptScore W1981422762C104317684 @default.
• W1981422762 hasConceptScore W1981422762C105580179 @default.
• W1981422762 hasConceptScore W1981422762C122871604 @default.
• W1981422762 hasConceptScore W1981422762C148762608 @default.
• W1981422762 hasConceptScore W1981422762C1491633281 @default.
• W1981422762 hasConceptScore W1981422762C153911025 @default.
• W1981422762 hasConceptScore W1981422762C169760540 @default.
• W1981422762 hasConceptScore W1981422762C2778993516 @default.
• W1981422762 hasConceptScore W1981422762C54009773 @default.
• W1981422762 hasConceptScore W1981422762C54355233 @default.
• W1981422762 hasConceptScore W1981422762C81885089 @default.
• W1981422762 hasConceptScore W1981422762C85789140 @default.
• W1981422762 hasConceptScore W1981422762C86803240 @default.
• W1981422762 hasConceptScore W1981422762C95444343 @default.
• W1981422762 hasIssue "5" @default.
• W1981422762 hasLocation W19814227621 @default.
• W1981422762 hasLocation W19814227622 @default.
• W1981422762 hasOpenAccess W1981422762 @default.
• W1981422762 hasPrimaryLocation W19814227621 @default.
• W1981422762 hasRelatedWork W1990669582 @default.
• W1981422762 hasRelatedWork W1993628232 @default.
• W1981422762 hasRelatedWork W2002689706 @default.
• W1981422762 hasRelatedWork W2044007342 @default.
• W1981422762 hasRelatedWork W2046793076 @default.
• W1981422762 hasRelatedWork W2067222264 @default.
• W1981422762 hasRelatedWork W2105741134 @default.
• W1981422762 hasRelatedWork W2406950557 @default.

• next