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• W1981429185 startingPage "1479" @default.
• W1981429185 abstract "Part of the nuclear ribosomal DNA unit of Pleurotus cornucopiae , including most of the intergenic sequences, was used as a probe to hybridize with DNA from eleven Pleurotus taxa (29 isolates), digested with nine restriction endonucleases. For all digests, a high level of rDNA heterogeneity between and within species was detected, which is in agreement with the phenetic variability already reported in previous studies on Pleurotus taxa. Numerical analysis of the results, performed by use of two different tree-making methods, clearly distinguished among well-defined species, i.e. P. dryinus, P. eryngii and P. flabellatus , which presented large phenetic distances with the rest of the taxa tested. P. abalonus , despite morphological similarity and interfertility with P. cystidiosus , appears to be a discrete taxon. Although they showed relative affinity, P. cornucopiae, P. columbinus and P. ostreatus presented large distances among their representative isolates, supporting the idea that they correspond to distinct taxa. All strains of P. pulmonarius, P. sajor-caju and P. sapidus having small phenetic distances were closely positioned on dendrograms; in relation to results from recent interfertility and isozyme studies, these results support the common genetic background of P. pulmonarius and P. sajor-caju ; the position of P. sapidus remains controversial. Furthermore, the rDNA analysis identified ten fragments suitable for species identification: eight allowed the characterization of five taxa ( P. cornucopiae. P. ostreatus, P. sajor-caju, P. abalonus and P. columbinus ) and two distinguished among clusters including related taxa. By a combination of just two restriction enzymes, the rDNA probe used seems to be the appropriate tool for both varietal characterization and protection of commercially valuable strains." @default.
• W1981429185 created "2016-06-24" @default.
• W1981429185 creator A5079612357 @default.
• W1981429185 creator A5084602829 @default.
• W1981429185 date "1995-06-01" @default.
• W1981429185 modified "2023-09-27" @default.
• W1981429185 title "Molecular systematics of the genus Pleurotus: analysis of restriction polymorphisms in ribosomal DNA" @default.
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• W1981429185 doi "https://doi.org/10.1099/13500872-141-6-1479" @default.
• W1981429185 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33799321" @default.
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