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• W1981434443 endingPage "105" @default.
• W1981434443 startingPage "96" @default.
• W1981434443 abstract "Capsaicinoid biosynthesis involves the participation of two substrates viz. vanillylamine and C9–C11 fatty acid moieties. Vanillylamine which is a derivative of vanillin is synthesized through a transaminase reaction in the phenylpropanoid pathway of capsaicinoid synthesis. Here we report the functional validation of earlier reported putative aminotransferase gene for vanillylamine biosynthesis in heterologous system using Agrobacterium mediated genetic transformation studies in Nicotiana tabacum and Capsicum frutescens calli cultures. Molecular analysis tools comprising PCR and Southern blot analysis have shown the integration of the foreign gene in N. tabacum and C. frutescens calli cultures. The study shows the production of vanillylamine in transformed N. tabacum callus cultures and also the reduction of vanillylamine production when whole gene based antisense binary vector construct was used in transformation of C. frutescens callus cultures. Vanillylamine production, aminotransferase assay with Western blot analysis for crude proteins of transformants established the production of putative aminotransferase (pAMT) protein in alternate plant. The result is a clear evidence of involvement of the reported putative aminotransferase responsible for vanillylamine biosynthesis in capsaicinoid biosynthesis pathway, confirming the gene function through functional validation." @default.
• W1981434443 created "2016-06-24" @default.
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• W1981434443 date "2012-10-01" @default.
• W1981434443 modified "2023-09-27" @default.
• W1981434443 title "Functional validation of Capsicum frutescens aminotransferase gene involved in vanillylamine biosynthesis using Agrobacterium mediated genetic transformation studies in Nicotiana tabacum and Capsicum frutescens calli cultures" @default.
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• W1981434443 doi "https://doi.org/10.1016/j.plantsci.2012.06.014" @default.
• W1981434443 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22921003" @default.
• W1981434443 hasPublicationYear "2012" @default.
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