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- W1981466964 abstract "Human and bovine RPE cells underwent changes in morphology and culture doubling times when passaged in serum-supplemented medium (CM). Furthermore, late passage human RPE cells subcultured in CM medium increased synthesis of three acidic, 43 000–63 000 D proteins. In order to provide a controlled environment for the study of RPE cells in vitro, we have devloped a method for growing human and bovine RPE in a serum-free defined medium (DM). RPE cells grown in DM required a 24 h pretreatment with CM to allow the cells to attach and spread on the substrate. Cells grown in DM retained an epithelioid morphology, a stable culture doubling time, and similar 2-D PAGE patterns through several subculturings." @default.
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- W1981466964 date "1984-10-01" @default.
- W1981466964 modified "2023-09-30" @default.
- W1981466964 title "A serum-free defined medium for retinal pigment epithelial cells" @default.
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- W1981466964 doi "https://doi.org/10.1016/0014-4827(84)90178-2" @default.
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