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• W1981480963 abstract "A dual channel, evanescent fluoroimmunoassay format is used to detect femtomolar analyte concentrations (i.e. less than 1 part per trillion [w/w]) on an etched channel siliconoxynitride thin film integrated optical waveguide. Two assays are used to demonstrate the dose-response behaviour of the sensor: (1) a direct assay of a fluorescently-labeled protein ligand binding to an immobilized protein receptor, and (2) an indirect sandwich assay of a non-fluorescent protein ligand binding to an immobilized protein receptor, as detected by the binding of a fluorescently-labeled secondary receptor protein. A red-emitting cyanine dye (Cy-5), which minimized background fluorescence and scatter losses of the waveguide, was used in both assays. To our knowledge, this is the first report of femtomolar sensitivity in an immunosensing instrument." @default.
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• W1981480963 date "1996-01-01" @default.
• W1981480963 modified "2023-10-11" @default.
• W1981480963 title "Femtomolar sensitivity using a channel-etched thin film waveguide fluoroimmunosensor" @default.
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• W1981480963 doi "https://doi.org/10.1016/0956-5663(96)83722-5" @default.
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