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- W1981567275 abstract "A flow-through real-time PCR system has been developed. PCR reagents were pumpedinto a borosilicate reaction chamber and sealed by the pump and a valve prior to thermal cycling.The system successfully reached temperature setpoints within 2°C and was able to measurefluorescence as a function of cycle number. The flow-through PCR system results were comparedwith results from a commercial real-time PCR machine. In both systems, primer dimers were formedin negative control reactions. The commercial instrument formed a product with the expected meltingtemperature with extracted Salmonella enterica DNA as the target while the flow-through PCRsystem formed a primer dimer with the extracted DNA sample. The cause of the unexpected primerdimer formation is being investigated." @default.
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- W1981567275 date "2004-01-01" @default.
- W1981567275 modified "2023-09-23" @default.
- W1981567275 title "Automated Real-Time PCR Biosensor for the Detection of Pathogens in Produce Irrigation Water" @default.
- W1981567275 doi "https://doi.org/10.13031/2013.17123" @default.
- W1981567275 hasPublicationYear "2004" @default.
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