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• W1981587516 abstract "Incorporation of intercellular adhesion molecule 1 (ICAM-1) into HIV-1 particles is known to markedly enhance the virus binding and infection of cells expressing lymphocyte function-associated antigen-1 (LFA-1). At the same time, ICAM-1 has been reported to exert a less pronounced effect on HIV-1 fusion with lymphoid cells. Here we examined the role of ICAM-1/LFA-1 interactions in productive HIV-1 entry into lymphoid cells using a direct virus-cell fusion assay. ICAM-1 promoted HIV-1 attachment to cells in a temperature-dependent manner. It exerted a marginal effect on virus binding in the cold, but enhanced binding up to 4-fold at physiological temperature. ICAM-1-independent attachment in the cold was readily reversible upon subsequent incubation at elevated temperature, whereas ICAM-1-bearing particles were largely retained by cells. The better virus retention resulted in a proportional increase in HIV-1 internalization and fusion, suggesting that ICAM-1 did not specifically accelerate endocytosis or fusion steps. We also measured the rates of CD4 engagement, productive endocytosis and HIV-endosome fusion using specific fusion inhibitors. These rates were virtually independent of the presence of ICAM-1 in viral particles. Importantly, irrespective of the presence of ICAM-1, HIV-1 escaped from the low temperature block, which stopped virus endocytosis and fusion, much later than from a membrane-impermeant fusion inhibitor targeting surface-accessible particles. This result, along with the complete inhibition of HIV-1 fusion by a small molecule dynamin inhibitor, implies this virus enters lymphoid cells used in this study via endocytosis and that this pathway is not altered by the viral ICAM-1. Our data highlight the role of ICAM-1 in stabilizing the HIV-1 attachment to LFA-1 expressing cells, which leads to a proportional enhancement of the receptor-mediated uptake and fusion with endosomes." @default.
• W1981587516 created "2016-06-24" @default.
• W1981587516 creator A5038256820 @default.
• W1981587516 creator A5054427560 @default.
• W1981587516 date "2012-09-06" @default.
• W1981587516 modified "2023-09-26" @default.
• W1981587516 title "Intercellular Adhesion Molecule 1 Promotes HIV-1 Attachment but Not Fusion to Target Cells" @default.
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• W1981587516 doi "https://doi.org/10.1371/journal.pone.0044827" @default.
• W1981587516 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3435301" @default.
• W1981587516 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22970312" @default.
• W1981587516 hasPublicationYear "2012" @default.
• W1981587516 type Work @default.
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