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- W1981749461 abstract "Numerical calculations, using Poisson-Boltzmann (PB) and counterion condensation (CC) polyelectrolyte theories, of the electrostatic free energy difference, DeltaGel, between single-stranded (coil) and double-helical DNA have been performed for solutions of NaDNA + NaCl with and without added MgCl2. Calculations have been made for conditions relevant to systems where experimental values of helix coil transition temperature (Tm) and other thermodynamic quantities have been measured. Comparison with experimental data has been possible by invoking values of Tm for solutions containing NaCl salt only. Resulting theoretical values of enthalpy, entropy, and heat capacity (for NaCl salt-containing solutions) and of Tm as a function of NaCl concentration in NaCl + MgCl2 solutions have thus been obtained. Qualitative and, to a large extent, quantitative reproduction of the experimental Tm, DeltaHm, DeltaSm, and DeltaCp values have been found from the results of polyelectrolyte theories. However, the quantitative resemblance of experimental data is considerably better for PB theory as compared to the CC model. Furthermore, some rather implausible qualitative conclusions are obtained within the CC results for DNA melting in NaCl + MgCl2 solutions. Our results argue in favor of the Poisson-Boltzmann theory, as compared to the counterion condensation theory." @default.
- W1981749461 created "2016-06-24" @default.
- W1981749461 creator A5001849365 @default.
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- W1981749461 date "1998-12-01" @default.
- W1981749461 modified "2023-10-17" @default.
- W1981749461 title "Application of Polyelectrolyte Theories for Analysis of DNA Melting in the Presence of Na+ and Mg2+ Ions" @default.
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- W1981749461 doi "https://doi.org/10.1016/s0006-3495(98)77745-8" @default.
- W1981749461 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1299975" @default.
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