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- W1981808770 abstract "To expand the spectroscopic utility of the non-natural amino acid p-cyanophenylalanine (PheCN), we examine the quenching efficiencies of a series of commonly encountered anions toward its fluorescence. We find that iodide exhibits an unusually large Stern-Volmer quenching constant, making it a convenient choice in PheCN fluorescence quenching studies. Indeed, using the villin headpiece subdomain as a testbed we demonstrate that iodide quenching of PheCN fluorescence offers a convenient means to reveal protein conformational heterogeneity. Furthermore, we show that the amino group of PheCN strongly quenches its fluorescence, suggesting that PheCN could be used as a local pH sensor." @default.
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- W1981808770 date "2013-03-01" @default.
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- W1981808770 title "Quenching of p-cyanophenylalanine fluorescence by various anions" @default.
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- W1981808770 doi "https://doi.org/10.1016/j.cplett.2013.02.015" @default.
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