FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1981822738> ?p ?o ?g. }

• W1981822738 endingPage "443" @default.
• W1981822738 startingPage "430" @default.
• W1981822738 abstract "Insulin-degrading enzyme (IDE) is involved in the clearance of many bioactive peptide substrates, including insulin and amyloid-β, peptides vital to the development of diabetes and Alzheimer's disease, respectively. IDE can also rapidly degrade hormones that are held together by intramolecular disulfide bond(s) without their reduction. Furthermore, IDE exhibits a remarkable ability to preferentially degrade structurally similar peptides such as the selective degradation of insulin-like growth factor (IGF)-II and transforming growth factor-α (TGF-α) over IGF-I and epidermal growth factor, respectively. Here, we used high-accuracy mass spectrometry to identify the cleavage sites of human IGF-II, TGF-α, amylin, reduced amylin, and amyloid-β by human IDE. We also determined the structures of human IDE–IGF-II and IDE–TGF-α at 2.3 Å and IDE–amylin at 2.9 Å. We found that IDE cleaves its substrates at multiple sites in a biased stochastic manner. Furthermore, the presence of a disulfide bond in amylin allows IDE to cut at an additional site in the middle of the peptide (amino acids 18–19). Our amylin-bound IDE structure offers insight into how the structural constraint from a disulfide bond in amylin can alter IDE cleavage sites. Together with NMR structures of amylin and the IGF and epidermal growth factor families, our work also reveals the structural basis of how the high dipole moment of substrates complements the charge distribution of the IDE catalytic chamber for the substrate selectivity. In addition, we show how the ability of substrates to properly anchor their N-terminus to the exosite of IDE and undergo a conformational switch upon binding to the catalytic chamber of IDE can also contribute to the selective degradation of structurally related growth factors." @default.
• W1981822738 created "2016-06-24" @default.
• W1981822738 creator A5013607512 @default.
• W1981822738 creator A5031289219 @default.
• W1981822738 creator A5033409338 @default.
• W1981822738 creator A5043432385 @default.
• W1981822738 creator A5070423321 @default.
• W1981822738 date "2010-01-01" @default.
• W1981822738 modified "2023-10-15" @default.
• W1981822738 title "Molecular Basis for the Recognition and Cleavages of IGF-II, TGF-α, and Amylin by Human Insulin-Degrading Enzyme" @default.
• W1981822738 cites W133843869 @default.
• W1981822738 cites W184255001 @default.
• W1981822738 cites W1967239474 @default.
• W1981822738 cites W1971855284 @default.
• W1981822738 cites W1972465684 @default.
• W1981822738 cites W1976901977 @default.
• W1981822738 cites W1992913621 @default.
• W1981822738 cites W1993630271 @default.
• W1981822738 cites W1995017064 @default.
• W1981822738 cites W1995541462 @default.
• W1981822738 cites W1996149691 @default.
• W1981822738 cites W2000842689 @default.
• W1981822738 cites W2007899228 @default.
• W1981822738 cites W2021624791 @default.
• W1981822738 cites W2025143098 @default.
• W1981822738 cites W2026413371 @default.
• W1981822738 cites W2029582401 @default.
• W1981822738 cites W2033309752 @default.
• W1981822738 cites W2036371154 @default.
• W1981822738 cites W2038663652 @default.
• W1981822738 cites W2038840577 @default.
• W1981822738 cites W2047608438 @default.
• W1981822738 cites W2053016048 @default.
• W1981822738 cites W2053684471 @default.
• W1981822738 cites W2056034415 @default.
• W1981822738 cites W2056838602 @default.
• W1981822738 cites W2063014525 @default.
• W1981822738 cites W2076517751 @default.
• W1981822738 cites W2089171551 @default.
• W1981822738 cites W2090284620 @default.
• W1981822738 cites W2092545648 @default.
• W1981822738 cites W2096457861 @default.
• W1981822738 cites W2103474552 @default.
• W1981822738 cites W2103794058 @default.
• W1981822738 cites W2120197945 @default.
• W1981822738 cites W2133796370 @default.
• W1981822738 cites W2137204463 @default.
• W1981822738 cites W2138997437 @default.
• W1981822738 cites W2144081223 @default.
• W1981822738 cites W2147177696 @default.
• W1981822738 doi "https://doi.org/10.1016/j.jmb.2009.10.072" @default.
• W1981822738 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2813390" @default.
• W1981822738 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19896952" @default.
• W1981822738 hasPublicationYear "2010" @default.
• W1981822738 type Work @default.
• W1981822738 sameAs 1981822738 @default.
• W1981822738 citedByCount "70" @default.
• W1981822738 countsByYear W19818227382012 @default.
• W1981822738 countsByYear W19818227382013 @default.
• W1981822738 countsByYear W19818227382014 @default.
• W1981822738 countsByYear W19818227382015 @default.
• W1981822738 countsByYear W19818227382016 @default.
• W1981822738 countsByYear W19818227382017 @default.
• W1981822738 countsByYear W19818227382018 @default.
• W1981822738 countsByYear W19818227382019 @default.
• W1981822738 countsByYear W19818227382020 @default.
• W1981822738 countsByYear W19818227382021 @default.
• W1981822738 countsByYear W19818227382022 @default.
• W1981822738 countsByYear W19818227382023 @default.
• W1981822738 crossrefType "journal-article" @default.
• W1981822738 hasAuthorship W1981822738A5013607512 @default.
• W1981822738 hasAuthorship W1981822738A5031289219 @default.
• W1981822738 hasAuthorship W1981822738A5033409338 @default.
• W1981822738 hasAuthorship W1981822738A5043432385 @default.
• W1981822738 hasAuthorship W1981822738A5070423321 @default.
• W1981822738 hasBestOaLocation W19818227382 @default.
• W1981822738 hasConcept C134018914 @default.
• W1981822738 hasConcept C151730666 @default.
• W1981822738 hasConcept C165220095 @default.
• W1981822738 hasConcept C170493617 @default.
• W1981822738 hasConcept C175156509 @default.
• W1981822738 hasConcept C179104552 @default.
• W1981822738 hasConcept C181199279 @default.
• W1981822738 hasConcept C185592680 @default.
• W1981822738 hasConcept C2775960820 @default.
• W1981822738 hasConcept C2776362946 @default.
• W1981822738 hasConcept C2777633098 @default.
• W1981822738 hasConcept C2779281246 @default.
• W1981822738 hasConcept C2779306644 @default.
• W1981822738 hasConcept C2780331951 @default.
• W1981822738 hasConcept C2780705028 @default.
• W1981822738 hasConcept C43369102 @default.
• W1981822738 hasConcept C55493867 @default.
• W1981822738 hasConcept C7860815 @default.
• W1981822738 hasConcept C86803240 @default.
• W1981822738 hasConceptScore W1981822738C134018914 @default.
• W1981822738 hasConceptScore W1981822738C151730666 @default.
• W1981822738 hasConceptScore W1981822738C165220095 @default.

• next