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• W1981952854 abstract "We have cloned a full‐length cDNA from Dictyostelium discoideum which encodes a new Ca 2+ ‐binding protein. The deduced protein (termed CBP1) is composed of 156 amino acids and contains four consensus metal‐ligating loop sequences found in helix‐loop‐helix motifs of many Ca 2+ ‐binding proteins. When expressed in bacteria as a GST fusion protein, CBP1 binds Ca 2+ in a 45 Ca 2+ overlay assay. CBP1 exhibits little amino acid sequence homology with Dictyostelium calmodulin or calfumirin‐1 (CAF‐1) except in the putative Ca 2+ ‐binding regions. Moreover, unlike calmodulin and CAF‐1 expression, CBP1 mRNA is expressed preferentially during the multicellular stages of development." @default.
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• W1981952854 date "1995-04-10" @default.
• W1981952854 modified "2023-10-09" @default.
• W1981952854 title "Cloning and expression in <i>Escherichia coli</i> of a cDNA encoding a developmentally regulated Ca²⁺‐binding protein from <i>Dictyostelium discoideum</i>" @default.
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• W1981952854 doi "https://doi.org/10.1016/0014-5793(95)00272-b" @default.
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