Matches in SemOpenAlex for { <https://semopenalex.org/work/W1982023581> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W1982023581 endingPage "580a" @default.
- W1982023581 startingPage "580a" @default.
- W1982023581 abstract "BipA is a highly conserved prokaryotic GTPase that functions as a master regulator of stress and virulence processes in bacteria. It is a member of the translational factor family of GTPases along with EF-G, IF-2 and LepA. Structural and biochemical data suggest that ribosome binding specificity for each member of this family lies in an effector domain. As with other bacterial GTPases, the ribosome binding and GTPase activities of this protein are tightly coupled. However, the mechanism by which this occurs is still unknown. A series of experiments have been designed to probe structural features of the protein to see if we can pinpoint specific areas of BipA, perhaps even individual residues, which are important to its association with the ribosome. Included in the list are the C-terminal effector domain of the protein, which is distinct to the BipA family of proteins, and amino acid residues in the switch I and II regions of the G domain. Using sucrose density gradients, we have shown that the C-terminal domain is required in order for BipA to bind to the ribosome. Moreover, deletion of this domain increases the GTP hydrolysis rates of the protein, likely through relief of inhibitory contacts. Additional evidencemore » has revealed an allosteric connection between the conformationally flexible switch II region and the C-terminal domain of BipA. Site directed mutagenesis, sucrose gradients and malachite green assays are being used to elucidate the details of this coupling.« less" @default.
- W1982023581 created "2016-06-24" @default.
- W1982023581 creator A5012359518 @default.
- W1982023581 creator A5032567081 @default.
- W1982023581 creator A5091638725 @default.
- W1982023581 date "2009-02-01" @default.
- W1982023581 modified "2023-09-29" @default.
- W1982023581 title "An Allosteric Pathway Revealed in the Ribosome Binding Stress Factor BipA" @default.
- W1982023581 doi "https://doi.org/10.1016/j.bpj.2008.12.3035" @default.
- W1982023581 hasPublicationYear "2009" @default.
- W1982023581 type Work @default.
- W1982023581 sameAs 1982023581 @default.
- W1982023581 citedByCount "0" @default.
- W1982023581 crossrefType "journal-article" @default.
- W1982023581 hasAuthorship W1982023581A5012359518 @default.
- W1982023581 hasAuthorship W1982023581A5032567081 @default.
- W1982023581 hasAuthorship W1982023581A5091638725 @default.
- W1982023581 hasBestOaLocation W19820235811 @default.
- W1982023581 hasConcept C104317684 @default.
- W1982023581 hasConcept C105580179 @default.
- W1982023581 hasConcept C113027372 @default.
- W1982023581 hasConcept C118716 @default.
- W1982023581 hasConcept C149364088 @default.
- W1982023581 hasConcept C163181125 @default.
- W1982023581 hasConcept C166342909 @default.
- W1982023581 hasConcept C181199279 @default.
- W1982023581 hasConcept C185592680 @default.
- W1982023581 hasConcept C207332259 @default.
- W1982023581 hasConcept C512185932 @default.
- W1982023581 hasConcept C51785407 @default.
- W1982023581 hasConcept C55493867 @default.
- W1982023581 hasConcept C62478195 @default.
- W1982023581 hasConcept C67705224 @default.
- W1982023581 hasConcept C80631254 @default.
- W1982023581 hasConcept C86803240 @default.
- W1982023581 hasConcept C88478588 @default.
- W1982023581 hasConcept C95444343 @default.
- W1982023581 hasConceptScore W1982023581C104317684 @default.
- W1982023581 hasConceptScore W1982023581C105580179 @default.
- W1982023581 hasConceptScore W1982023581C113027372 @default.
- W1982023581 hasConceptScore W1982023581C118716 @default.
- W1982023581 hasConceptScore W1982023581C149364088 @default.
- W1982023581 hasConceptScore W1982023581C163181125 @default.
- W1982023581 hasConceptScore W1982023581C166342909 @default.
- W1982023581 hasConceptScore W1982023581C181199279 @default.
- W1982023581 hasConceptScore W1982023581C185592680 @default.
- W1982023581 hasConceptScore W1982023581C207332259 @default.
- W1982023581 hasConceptScore W1982023581C512185932 @default.
- W1982023581 hasConceptScore W1982023581C51785407 @default.
- W1982023581 hasConceptScore W1982023581C55493867 @default.
- W1982023581 hasConceptScore W1982023581C62478195 @default.
- W1982023581 hasConceptScore W1982023581C67705224 @default.
- W1982023581 hasConceptScore W1982023581C80631254 @default.
- W1982023581 hasConceptScore W1982023581C86803240 @default.
- W1982023581 hasConceptScore W1982023581C88478588 @default.
- W1982023581 hasConceptScore W1982023581C95444343 @default.
- W1982023581 hasIssue "3" @default.
- W1982023581 hasLocation W19820235811 @default.
- W1982023581 hasOpenAccess W1982023581 @default.
- W1982023581 hasPrimaryLocation W19820235811 @default.
- W1982023581 hasRelatedWork W1486164103 @default.
- W1982023581 hasRelatedWork W1964692981 @default.
- W1982023581 hasRelatedWork W1988803183 @default.
- W1982023581 hasRelatedWork W1992031613 @default.
- W1982023581 hasRelatedWork W1994394909 @default.
- W1982023581 hasRelatedWork W2000174017 @default.
- W1982023581 hasRelatedWork W2006313129 @default.
- W1982023581 hasRelatedWork W2014648997 @default.
- W1982023581 hasRelatedWork W2024305595 @default.
- W1982023581 hasRelatedWork W2029807656 @default.
- W1982023581 hasRelatedWork W2030764144 @default.
- W1982023581 hasRelatedWork W2034687379 @default.
- W1982023581 hasRelatedWork W2039175133 @default.
- W1982023581 hasRelatedWork W2042674021 @default.
- W1982023581 hasRelatedWork W2080108346 @default.
- W1982023581 hasRelatedWork W2116152709 @default.
- W1982023581 hasRelatedWork W2146674824 @default.
- W1982023581 hasRelatedWork W2274505228 @default.
- W1982023581 hasRelatedWork W2591182649 @default.
- W1982023581 hasRelatedWork W824155577 @default.
- W1982023581 hasVolume "96" @default.
- W1982023581 isParatext "false" @default.
- W1982023581 isRetracted "false" @default.
- W1982023581 magId "1982023581" @default.
- W1982023581 workType "article" @default.