FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1982033373> ?p ?o ?g. }

• W1982033373 endingPage "69" @default.
• W1982033373 startingPage "58" @default.
• W1982033373 abstract "To characterize a proprietary therapeutic monoclonal antibody (mAb) candidate, a rigorous biophysical study consisting of 53 Biacore and kinetic exclusion assay (KinExA) experiments was undertaken on the therapeutic mAb complexing with its target antigen. Unexpectedly, the observed binding kinetics depended on the chip used, suggesting that the negatively charged carboxyl groups on CM5, CM4, and C1 chips were adversely affecting the Biacore kinetic results. To study this hypothesis, Biacore solution-phase and KinExA equilibrium titrations, as well as KinExA kinetic measurements, were performed to establish accurate values for the affinity and kinetic rate constants of the binding reaction between antigen and mAb. The results revealed that as the negative charge on the biosensor surface decreased, the binding kinetics and KD approached the accurate binding parameters more closely when measured in solution. Two potential causes for the artifactual Biacore surface-based measurements are (i) steric hindrance of antigen binding arising from an interaction of the negatively charged carboxymethyldextran matrix with the mAb, which is a highly basic protein with a pI of 9.4, and (ii) an electrostatic repulsion between the negatively charged antigen and the carboxymethyldextran matrix. Importantly, simple diagnostic tests can be performed early in the measurement process to identify these types of matrix-mediated artifacts." @default.
• W1982033373 created "2016-06-24" @default.
• W1982033373 creator A5009488234 @default.
• W1982033373 creator A5030976019 @default.
• W1982033373 creator A5050120906 @default.
• W1982033373 creator A5057250302 @default.
• W1982033373 creator A5066137013 @default.
• W1982033373 creator A5088721726 @default.
• W1982033373 date "2012-10-01" @default.
• W1982033373 modified "2023-09-26" @default.
• W1982033373 title "Biacore surface matrix effects on the binding kinetics and affinity of an antigen/antibody complex" @default.
• W1982033373 cites W1929229765 @default.
• W1982033373 cites W1976705206 @default.
• W1982033373 cites W1981902013 @default.
• W1982033373 cites W1983214653 @default.
• W1982033373 cites W2005895326 @default.
• W1982033373 cites W2010801138 @default.
• W1982033373 cites W2011594810 @default.
• W1982033373 cites W2023594790 @default.
• W1982033373 cites W2034471601 @default.
• W1982033373 cites W2036753716 @default.
• W1982033373 cites W2043278285 @default.
• W1982033373 cites W2043574666 @default.
• W1982033373 cites W2048999801 @default.
• W1982033373 cites W2059639914 @default.
• W1982033373 cites W2063062799 @default.
• W1982033373 cites W2067547649 @default.
• W1982033373 cites W2079628431 @default.
• W1982033373 cites W2083908960 @default.
• W1982033373 cites W2087767169 @default.
• W1982033373 cites W2137228190 @default.
• W1982033373 cites W2156294304 @default.
• W1982033373 cites W2162298868 @default.
• W1982033373 cites W2164929179 @default.
• W1982033373 doi "https://doi.org/10.1016/j.ab.2012.06.024" @default.
• W1982033373 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22766435" @default.
• W1982033373 hasPublicationYear "2012" @default.
• W1982033373 type Work @default.
• W1982033373 sameAs 1982033373 @default.
• W1982033373 citedByCount "70" @default.
• W1982033373 countsByYear W19820333732012 @default.
• W1982033373 countsByYear W19820333732013 @default.
• W1982033373 countsByYear W19820333732014 @default.
• W1982033373 countsByYear W19820333732015 @default.
• W1982033373 countsByYear W19820333732016 @default.
• W1982033373 countsByYear W19820333732017 @default.
• W1982033373 countsByYear W19820333732018 @default.
• W1982033373 countsByYear W19820333732019 @default.
• W1982033373 countsByYear W19820333732020 @default.
• W1982033373 countsByYear W19820333732021 @default.
• W1982033373 countsByYear W19820333732022 @default.
• W1982033373 countsByYear W19820333732023 @default.
• W1982033373 crossrefType "journal-article" @default.
• W1982033373 hasAuthorship W1982033373A5009488234 @default.
• W1982033373 hasAuthorship W1982033373A5030976019 @default.
• W1982033373 hasAuthorship W1982033373A5050120906 @default.
• W1982033373 hasAuthorship W1982033373A5057250302 @default.
• W1982033373 hasAuthorship W1982033373A5066137013 @default.
• W1982033373 hasAuthorship W1982033373A5088721726 @default.
• W1982033373 hasConcept C106487976 @default.
• W1982033373 hasConcept C121332964 @default.
• W1982033373 hasConcept C12554922 @default.
• W1982033373 hasConcept C147483822 @default.
• W1982033373 hasConcept C147789679 @default.
• W1982033373 hasConcept C148898269 @default.
• W1982033373 hasConcept C152900378 @default.
• W1982033373 hasConcept C159654299 @default.
• W1982033373 hasConcept C170493617 @default.
• W1982033373 hasConcept C184866935 @default.
• W1982033373 hasConcept C185592680 @default.
• W1982033373 hasConcept C201194858 @default.
• W1982033373 hasConcept C203014093 @default.
• W1982033373 hasConcept C43617362 @default.
• W1982033373 hasConcept C542903549 @default.
• W1982033373 hasConcept C55493867 @default.
• W1982033373 hasConcept C62520636 @default.
• W1982033373 hasConcept C71240020 @default.
• W1982033373 hasConcept C86803240 @default.
• W1982033373 hasConceptScore W1982033373C106487976 @default.
• W1982033373 hasConceptScore W1982033373C121332964 @default.
• W1982033373 hasConceptScore W1982033373C12554922 @default.
• W1982033373 hasConceptScore W1982033373C147483822 @default.
• W1982033373 hasConceptScore W1982033373C147789679 @default.
• W1982033373 hasConceptScore W1982033373C148898269 @default.
• W1982033373 hasConceptScore W1982033373C152900378 @default.
• W1982033373 hasConceptScore W1982033373C159654299 @default.
• W1982033373 hasConceptScore W1982033373C170493617 @default.
• W1982033373 hasConceptScore W1982033373C184866935 @default.
• W1982033373 hasConceptScore W1982033373C185592680 @default.
• W1982033373 hasConceptScore W1982033373C201194858 @default.
• W1982033373 hasConceptScore W1982033373C203014093 @default.
• W1982033373 hasConceptScore W1982033373C43617362 @default.
• W1982033373 hasConceptScore W1982033373C542903549 @default.
• W1982033373 hasConceptScore W1982033373C55493867 @default.
• W1982033373 hasConceptScore W1982033373C62520636 @default.
• W1982033373 hasConceptScore W1982033373C71240020 @default.
• W1982033373 hasConceptScore W1982033373C86803240 @default.
• W1982033373 hasIssue "1" @default.

• next