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- W1982045581 abstract "Pig small intestinal mRNA was translated in a rabbit reticulocyte lysate system supplemented with microsomal membranes. Castanospermine, an inhibitor of glucosidase I, induced a high mannose-glycosylated form of microvillar aminopeptidase N(EC 3.4.11.2) of increased molecular mass, indicating the blocked removal of glucose residues. In contrast to its reduced expression in a mucosal explant system [(1986) Biochem. J. 240, 777–782], this molecular form of aminopeptidase N was at least as abundant in cell-free translation as its normal high mannose-glycosylated counterpart, ruling out degradation taking place in the rough endoplasmic reticulum. Degradation of newly produced malprocessed enzyme must therefore occur at a later stage during intracellular transport, presumably in the sarcoplasmic reticulum or in transitional elements between this organelle the Golgi complex." @default.
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- W1982045581 date "1988-02-08" @default.
- W1982045581 modified "2023-10-16" @default.
- W1982045581 title "Biosynthesis of intestinal microvillar proteins Effect of castanospermine on cell-free synthesis of aminopeptidase N" @default.
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- W1982045581 doi "https://doi.org/10.1016/0014-5793(88)80594-5" @default.
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