FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1982079062> ?p ?o ?g. }

• W1982079062 endingPage "446" @default.
• W1982079062 startingPage "438" @default.
• W1982079062 abstract "Enzyme kinetic plots relating the initial rate of activation of pro-urokinase to urokinase by plasmin, according to the concentration of substrate, were smooth downward curves and indicated that an apparent decrease in binding affinity occurred with increase in the concentration of pro-urokinase. Such nonlinear plots were obtained with plasmin 1 and also plasmin 2. Over sections of each curve it was possible to estimate apparent kinetic constants. At the uppermost concentrations of substrate tested, these were Km 2.9 microM and kcat 35.5 min-1 for plasmin 1, and at the lowermost concentrations, Km 9.5 nM and kcat 2.0 min-1. Linear plots were obtained when the single proteolytic cleavage was made by K5-plasmin or undegraded plasmin in the presence of 1.0 mM 6-aminohexanoic acid (6-AHa). Constants were estimated for catalysis of this reaction by K5 plasmin to be Km 6.0 microM and kcat 38 min-1 (r = 0.987). The catalytic efficiency of plasmin, at the lowermost concentrations of pro-urokinase tested, was therefore 33-fold higher than that of K5-plasmin. Plotting of data for the cleavage of pro-urokinase by plasmin 1 (in the absence of 6-AHa) according to the model of Hill, gave a slope of 0.5 at the lowermost concentrations of pro-urokinase increasing to 1.0 at higher concentrations (greater than 0.3 microM); such a profile is characteristic of negative cooperativity. The rates of formation of plasmin and urokinase in a mixture containing a low concentration of plasminogen and pro-urokinase were measured and compared to those predicted by a computer program designed to calculate theoretical rates using available kinetic data. The observed rates of generation of both plasmin and urokinase coincided to those predicted from the negative cooperativity model. The mechanism of the negative cooperativity may reside in a conformational change induced by binding of pro-urokinase to the kringle structure of plasmin. This property may be of significance in controlling the fibrinolytic properties of the urokinase-type plasminogen activator system." @default.
• W1982079062 created "2016-06-24" @default.
• W1982079062 creator A5001565434 @default.
• W1982079062 creator A5063047682 @default.
• W1982079062 creator A5068612576 @default.
• W1982079062 creator A5075795425 @default.
• W1982079062 date "1989-02-01" @default.
• W1982079062 modified "2023-09-25" @default.
• W1982079062 title "Activation of pro-urokinase by plasmin: Non-michaelian kinetics indicates a mechanism of negative cooperativity" @default.
• W1982079062 cites W1483611820 @default.
• W1982079062 cites W1493910160 @default.
• W1982079062 cites W1505423658 @default.
• W1982079062 cites W1510618279 @default.
• W1982079062 cites W1521047460 @default.
• W1982079062 cites W1523898622 @default.
• W1982079062 cites W1524135583 @default.
• W1982079062 cites W1534598094 @default.
• W1982079062 cites W1557927258 @default.
• W1982079062 cites W1583098109 @default.
• W1982079062 cites W1597299868 @default.
• W1982079062 cites W1604425981 @default.
• W1982079062 cites W1608281458 @default.
• W1982079062 cites W1627300959 @default.
• W1982079062 cites W1961340888 @default.
• W1982079062 cites W1965572453 @default.
• W1982079062 cites W1966087742 @default.
• W1982079062 cites W1987960186 @default.
• W1982079062 cites W2022761196 @default.
• W1982079062 cites W2043352260 @default.
• W1982079062 cites W2058530696 @default.
• W1982079062 cites W2067362956 @default.
• W1982079062 cites W2082553168 @default.
• W1982079062 cites W2091317550 @default.
• W1982079062 cites W2119837316 @default.
• W1982079062 cites W2156513784 @default.
• W1982079062 cites W2320417794 @default.
• W1982079062 cites W2395871307 @default.
• W1982079062 cites W2397491329 @default.
• W1982079062 cites W2466223857 @default.
• W1982079062 cites W303857720 @default.
• W1982079062 doi "https://doi.org/10.1016/0003-9861(89)90311-1" @default.
• W1982079062 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2521553" @default.
• W1982079062 hasPublicationYear "1989" @default.
• W1982079062 type Work @default.
• W1982079062 sameAs 1982079062 @default.
• W1982079062 citedByCount "24" @default.
• W1982079062 countsByYear W19820790622012 @default.
• W1982079062 countsByYear W19820790622023 @default.
• W1982079062 crossrefType "journal-article" @default.
• W1982079062 hasAuthorship W1982079062A5001565434 @default.
• W1982079062 hasAuthorship W1982079062A5063047682 @default.
• W1982079062 hasAuthorship W1982079062A5068612576 @default.
• W1982079062 hasAuthorship W1982079062A5075795425 @default.
• W1982079062 hasConcept C121332964 @default.
• W1982079062 hasConcept C12554922 @default.
• W1982079062 hasConcept C135889238 @default.
• W1982079062 hasConcept C148898269 @default.
• W1982079062 hasConcept C151730666 @default.
• W1982079062 hasConcept C166014724 @default.
• W1982079062 hasConcept C175156509 @default.
• W1982079062 hasConcept C181199279 @default.
• W1982079062 hasConcept C185592680 @default.
• W1982079062 hasConcept C18903297 @default.
• W1982079062 hasConcept C2776892390 @default.
• W1982079062 hasConcept C2777289219 @default.
• W1982079062 hasConcept C2781071845 @default.
• W1982079062 hasConcept C41183919 @default.
• W1982079062 hasConcept C43369102 @default.
• W1982079062 hasConcept C43617362 @default.
• W1982079062 hasConcept C54355233 @default.
• W1982079062 hasConcept C55493867 @default.
• W1982079062 hasConcept C56856141 @default.
• W1982079062 hasConcept C62520636 @default.
• W1982079062 hasConcept C71240020 @default.
• W1982079062 hasConcept C86803240 @default.
• W1982079062 hasConceptScore W1982079062C121332964 @default.
• W1982079062 hasConceptScore W1982079062C12554922 @default.
• W1982079062 hasConceptScore W1982079062C135889238 @default.
• W1982079062 hasConceptScore W1982079062C148898269 @default.
• W1982079062 hasConceptScore W1982079062C151730666 @default.
• W1982079062 hasConceptScore W1982079062C166014724 @default.
• W1982079062 hasConceptScore W1982079062C175156509 @default.
• W1982079062 hasConceptScore W1982079062C181199279 @default.
• W1982079062 hasConceptScore W1982079062C185592680 @default.
• W1982079062 hasConceptScore W1982079062C18903297 @default.
• W1982079062 hasConceptScore W1982079062C2776892390 @default.
• W1982079062 hasConceptScore W1982079062C2777289219 @default.
• W1982079062 hasConceptScore W1982079062C2781071845 @default.
• W1982079062 hasConceptScore W1982079062C41183919 @default.
• W1982079062 hasConceptScore W1982079062C43369102 @default.
• W1982079062 hasConceptScore W1982079062C43617362 @default.
• W1982079062 hasConceptScore W1982079062C54355233 @default.
• W1982079062 hasConceptScore W1982079062C55493867 @default.
• W1982079062 hasConceptScore W1982079062C56856141 @default.
• W1982079062 hasConceptScore W1982079062C62520636 @default.
• W1982079062 hasConceptScore W1982079062C71240020 @default.
• W1982079062 hasConceptScore W1982079062C86803240 @default.
• W1982079062 hasIssue "2" @default.

• next