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- W1982105410 abstract "Abstract The commercial preparation of pectinase (Pectinex Ultra SP‐L) was conjugated to alginate by noncovalent interactions by employing 1% alginate during the conjugation protocol. The optimum “immobilization efficiency” was 0.76. The pH optimum and the thermal stability of the enzyme remained unchanged upon conjugation with alginate. The soluble bioconjugate showed a 3‐fold increase in V max / K m as compared to the free enzyme when the smart biocatalyst was used for chitosan hydrolysis. Time course hydrolysis of chitosan thus showed higher conversion of chitosan into reducing oligosaccharides/sugars. The smart bioconjugate could be reused five times without any detectable loss of chitosanase activity." @default.
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- W1982105410 date "2003-01-01" @default.
- W1982105410 modified "2023-10-15" @default.
- W1982105410 title "A Smart Bioconjugate of Alginate and Pectinase with Unusual Biological Activity toward Chitosan" @default.
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- W1982105410 doi "https://doi.org/10.1021/bp034080x" @default.
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