Matches in SemOpenAlex for { <https://semopenalex.org/work/W1982138514> ?p ?o ?g. }
- W1982138514 endingPage "e0118063" @default.
- W1982138514 startingPage "e0118063" @default.
- W1982138514 abstract "The bacterial stringent response is triggered by deficiencies of available nutrients and other environmental stresses. It is mediated by 5'-triphosphate-guanosine-3'-diphosphate and 5'-diphosphate-guanosine-3'-diphosphate (collectively (p)ppGpp) and generates global changes in gene expression and metabolism that enable bacteria to adapt to and survive these challenges. Borrelia burgdorferi encounters multiple stressors in its cycling between ticks and mammals that could trigger the stringent response. We have previously shown that the B. burgdorferi stringent response is mediated by a single enzyme, RelBbu, with both (p)ppGpp synthase and hydrolase activities, and that a B. burgdorferi 297 relBbu null deletion mutant was defective in adapting to stationary phase, incapable of down-regulating synthesis of rRNA and could not infect mice. We have now used this deletion mutant and microarray analysis to identify genes comprising the rel regulon in B. burgdorferi cultured at 34°C, and found that transcription of genes involved in glycerol metabolism is induced by relBbu. Culture of the wild type parental strain, the relBbu deletion mutant and its complemented derivative at 34°C and 25°C in media containing glucose or glycerol as principal carbon sources revealed a growth defect in the mutant, most evident at the lower temperature. Transcriptional analysis of the glp operon for glycerol uptake and metabolism in these three strains confirmed that relBbu was necessary and sufficient to increase transcription of this operon in the presence of glycerol at both temperatures. These results confirm and extend previous findings regarding the stringent response in B. burgdorferi. They also demonstrate that the stringent response regulates glycerol metabolism in this organism and is likely crucial for its optimal growth in ticks." @default.
- W1982138514 created "2016-06-24" @default.
- W1982138514 creator A5004511171 @default.
- W1982138514 creator A5008931530 @default.
- W1982138514 creator A5022025616 @default.
- W1982138514 creator A5044091673 @default.
- W1982138514 creator A5062423650 @default.
- W1982138514 creator A5086165449 @default.
- W1982138514 creator A5086638695 @default.
- W1982138514 date "2015-02-17" @default.
- W1982138514 modified "2023-10-09" @default.
- W1982138514 title "Characterization of the RelBbu Regulon in Borrelia burgdorferi Reveals Modulation of Glycerol Metabolism by (p)ppGpp" @default.
- W1982138514 cites W1512671399 @default.
- W1982138514 cites W1640253646 @default.
- W1982138514 cites W1709473677 @default.
- W1982138514 cites W1917921565 @default.
- W1982138514 cites W1926303070 @default.
- W1982138514 cites W1946642412 @default.
- W1982138514 cites W1946717434 @default.
- W1982138514 cites W1964979713 @default.
- W1982138514 cites W1965070851 @default.
- W1982138514 cites W1965822637 @default.
- W1982138514 cites W1969973578 @default.
- W1982138514 cites W1970571665 @default.
- W1982138514 cites W1971866858 @default.
- W1982138514 cites W1974053222 @default.
- W1982138514 cites W1975853762 @default.
- W1982138514 cites W1982958351 @default.
- W1982138514 cites W1986842298 @default.
- W1982138514 cites W1994834789 @default.
- W1982138514 cites W2007322603 @default.
- W1982138514 cites W2013636685 @default.
- W1982138514 cites W2014754473 @default.
- W1982138514 cites W2016413218 @default.
- W1982138514 cites W2016763232 @default.
- W1982138514 cites W2023359102 @default.
- W1982138514 cites W2025191048 @default.
- W1982138514 cites W2034876354 @default.
- W1982138514 cites W2037310526 @default.
- W1982138514 cites W2039160511 @default.
- W1982138514 cites W2040386523 @default.
- W1982138514 cites W2040976109 @default.
- W1982138514 cites W2042976303 @default.
- W1982138514 cites W2043454090 @default.
- W1982138514 cites W2044171296 @default.
- W1982138514 cites W2044493726 @default.
- W1982138514 cites W2049296526 @default.
- W1982138514 cites W2057635344 @default.
- W1982138514 cites W2058395746 @default.
- W1982138514 cites W2059690875 @default.
- W1982138514 cites W2067092006 @default.
- W1982138514 cites W2073247834 @default.
- W1982138514 cites W2079580196 @default.
- W1982138514 cites W2081114359 @default.
- W1982138514 cites W2087099306 @default.
- W1982138514 cites W2089809246 @default.
- W1982138514 cites W2093772258 @default.
- W1982138514 cites W2093855789 @default.
- W1982138514 cites W2100234064 @default.
- W1982138514 cites W2101906088 @default.
- W1982138514 cites W2102154042 @default.
- W1982138514 cites W2102757151 @default.
- W1982138514 cites W2103087390 @default.
- W1982138514 cites W2105028012 @default.
- W1982138514 cites W2105691511 @default.
- W1982138514 cites W2105786825 @default.
- W1982138514 cites W2106681306 @default.
- W1982138514 cites W2112864683 @default.
- W1982138514 cites W2120021098 @default.
- W1982138514 cites W2123384320 @default.
- W1982138514 cites W2125133724 @default.
- W1982138514 cites W2125616096 @default.
- W1982138514 cites W2125809659 @default.
- W1982138514 cites W2126588333 @default.
- W1982138514 cites W2131596454 @default.
- W1982138514 cites W2132657962 @default.
- W1982138514 cites W2132959045 @default.
- W1982138514 cites W2134269094 @default.
- W1982138514 cites W2136252131 @default.
- W1982138514 cites W2136848251 @default.
- W1982138514 cites W2137483855 @default.
- W1982138514 cites W2138611595 @default.
- W1982138514 cites W2139252483 @default.
- W1982138514 cites W2140211022 @default.
- W1982138514 cites W2142899232 @default.
- W1982138514 cites W2143334851 @default.
- W1982138514 cites W2148111565 @default.
- W1982138514 cites W2155142409 @default.
- W1982138514 cites W2156420781 @default.
- W1982138514 cites W2157570019 @default.
- W1982138514 cites W2159319229 @default.
- W1982138514 cites W2162181580 @default.
- W1982138514 cites W2162380932 @default.
- W1982138514 cites W2166799325 @default.
- W1982138514 cites W2167836254 @default.
- W1982138514 cites W2170069130 @default.
- W1982138514 cites W2171451207 @default.
- W1982138514 cites W2172147867 @default.