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- W1982148617 abstract "The sera of 49 subjects with IgG, IgA or IgM benign monoclonal gammopathy-components were examined by agarose gel isoelectric focusing followed by Coomassie Brilliant Blue R-250 staining as well as avidin-biotin amplified double-antibody peroxidase labelling after nitrocellulose blotting. IgG-components gave 2–10 distinct bands within the isoelectric point (pI)-range of pH 6.5–9.5, IgA-components were focused into 10–15 bands with pI-values of pH 4.5–6.5 and the IgM-components gave 1–2 fractions within the pI-range of pH 4.5–6.5. The avidin-biotin peroxidase complex (ABC) technique exhibited some tendency to non-specific labeling related to the avidin-biotin component and depending on the relative amounts of the individual proteins. However, the method has a definite potential for the visualization of submicrogram quantities of immunoglobulins blotted to nitrocellulose membranes after isoelectric focusing." @default.
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- W1982148617 date "1985-01-01" @default.
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- W1982148617 title "Isoelectric focusing of monoclonal immunoglobulin G, A and M followed by detection with the avidin-biotin system" @default.
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- W1982148617 doi "https://doi.org/10.1002/elps.1150060305" @default.
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