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• W1982149924 endingPage "178" @default.
• W1982149924 startingPage "157" @default.
• W1982149924 abstract "Advances are needed in the site-directed mutagenesis of large plasmids for protein structure-function studies, as current methods are often inefficient, complicated and time-consuming. Here two new methods are reported that overcome these difficulties, namely the single primer extension reaction (SSPER) strategy that reaches 100% efficiency and the reduce recycle PCR (rrPCR) method that is advantageous in generating single and pairwise combinations of mutations. Both methods are distinguished from current technologies by the addition of a step that easily removes the oligonucleotide primer(s) after the first reaction, thus allowing for the addition of a second reaction in chronological sequence to generate and isolate the appropriate DNA product with the site-directed mutation(s). High efficiency of the methods is demonstrated by generating single and paired combinations of the 11 site-directed mutations targeted on 5 different plasmid DNA templates ranging from 10 to 12 kb and 57–60% GC-content at a rate of 50–100%. Overall, the methods are demonstrated to be (i) highly accurate, allowing for screening of plasmids by DNA sequencing, (ii) streamlined to generate the mutations within a single day, (iii) cost-effective in requiring only two primers and two enzymes (DpnI and a proofreading DNA polymerase), (iv) straightforward in primer design, (v) applicable for both large and small plasmids, and (vi) easily implemented by entry level researchers." @default.
• W1982149924 created "2016-06-24" @default.
• W1982149924 creator A5069076893 @default.
• W1982149924 creator A5082233759 @default.
• W1982149924 date "1997-12-01" @default.
• W1982149924 modified "2023-10-17" @default.
• W1982149924 title "Approaches to DNA Mutagenesis: An Overview" @default.
• W1982149924 cites W1024346071 @default.
• W1982149924 cites W1431773207 @default.
• W1982149924 cites W1516550690 @default.
• W1982149924 cites W1561041749 @default.
• W1982149924 cites W1563608009 @default.
• W1982149924 cites W1600531441 @default.
• W1982149924 cites W1601687245 @default.
• W1982149924 cites W1951055954 @default.
• W1982149924 cites W1964449641 @default.
• W1982149924 cites W1964931822 @default.
• W1982149924 cites W1965498454 @default.
• W1982149924 cites W1966030834 @default.
• W1982149924 cites W1967006909 @default.
• W1982149924 cites W1969563247 @default.
• W1982149924 cites W1971878420 @default.
• W1982149924 cites W1972183072 @default.
• W1982149924 cites W1972281375 @default.
• W1982149924 cites W1972626408 @default.
• W1982149924 cites W1975593876 @default.
• W1982149924 cites W1977284764 @default.
• W1982149924 cites W1977836925 @default.
• W1982149924 cites W1978754461 @default.
• W1982149924 cites W1979291805 @default.
• W1982149924 cites W1981535644 @default.
• W1982149924 cites W1981765089 @default.
• W1982149924 cites W1982731004 @default.
• W1982149924 cites W1984545965 @default.
• W1982149924 cites W1986289016 @default.
• W1982149924 cites W1986603376 @default.
• W1982149924 cites W1987666378 @default.
• W1982149924 cites W1988037036 @default.
• W1982149924 cites W1991339183 @default.
• W1982149924 cites W1991731715 @default.
• W1982149924 cites W1991770249 @default.
• W1982149924 cites W1993084901 @default.
• W1982149924 cites W1994809213 @default.
• W1982149924 cites W1995263127 @default.
• W1982149924 cites W1995352629 @default.
• W1982149924 cites W1997049147 @default.
• W1982149924 cites W1998029045 @default.
• W1982149924 cites W2000089273 @default.
• W1982149924 cites W2000976347 @default.
• W1982149924 cites W2001754861 @default.
• W1982149924 cites W2001934760 @default.
• W1982149924 cites W2003843687 @default.
• W1982149924 cites W2006664630 @default.
• W1982149924 cites W2006762876 @default.
• W1982149924 cites W2007425170 @default.
• W1982149924 cites W2011679894 @default.
• W1982149924 cites W2012730350 @default.
• W1982149924 cites W2014199308 @default.
• W1982149924 cites W2015193101 @default.
• W1982149924 cites W2016718065 @default.
• W1982149924 cites W2017140473 @default.
• W1982149924 cites W2017193974 @default.
• W1982149924 cites W2018669799 @default.
• W1982149924 cites W2021225519 @default.
• W1982149924 cites W2024056386 @default.
• W1982149924 cites W2024147368 @default.
• W1982149924 cites W2024470778 @default.
• W1982149924 cites W2027286798 @default.
• W1982149924 cites W2028459708 @default.
• W1982149924 cites W2029097097 @default.
• W1982149924 cites W2034699600 @default.
• W1982149924 cites W2035923281 @default.
• W1982149924 cites W2036451343 @default.
• W1982149924 cites W2037411762 @default.
• W1982149924 cites W2040061707 @default.
• W1982149924 cites W2040198458 @default.
• W1982149924 cites W2040718787 @default.
• W1982149924 cites W2040960678 @default.
• W1982149924 cites W2042655455 @default.
• W1982149924 cites W2044589156 @default.
• W1982149924 cites W2045916068 @default.
• W1982149924 cites W2047472824 @default.
• W1982149924 cites W2048117183 @default.
• W1982149924 cites W2048490575 @default.
• W1982149924 cites W2049239988 @default.
• W1982149924 cites W2050595579 @default.
• W1982149924 cites W2053362861 @default.
• W1982149924 cites W2054919238 @default.
• W1982149924 cites W2055357983 @default.
• W1982149924 cites W2055464292 @default.
• W1982149924 cites W2055755865 @default.
• W1982149924 cites W2060261032 @default.
• W1982149924 cites W2060913542 @default.
• W1982149924 cites W2061038863 @default.
• W1982149924 cites W2061338529 @default.
• W1982149924 cites W2063459461 @default.
• W1982149924 cites W2064504648 @default.
• W1982149924 cites W2066618017 @default.

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