FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1982160785> ?p ?o ?g. }

• W1982160785 endingPage "268" @default.
• W1982160785 startingPage "251" @default.
• W1982160785 abstract "A number of substances commonly found in solutions in which deoxyribonucleic acid (DNA) is to be measured were tested for their effects on the color developed during the assay for DNA by methods using the indole, diphenylamine, and cysteine reactions. Mercaptoethanol and dithiothreitol produced marked changes in the color developed by all the reactions. Other compounds which seriously affected the color developed by one or more of the reactions included sodium citrate, tris(hydroxymethyl)aminomethane, ethylenediaminetetraacetate, sodium nitrate, sodium phosphate, and ammonium sulfate. An equation was derived which relates the absorbance that is observed in the presence of a known concentration of an interfering substance to the absorbance that would be observed in its absence. The constants necessary for solving this equation were determined for 18 substances that interfere with one or more of the reactions studied. The range of concentrations of DNA over which Beer's law applies, and therefore over which the equation is valid, was estimated for some of the substances tested. The data obtained for this study were also used to estimate the precision and sensitivity of these colorimetric methods. The Dische diphenylamine method was found to provide the best combination of precision and ease of performance for many routine purposes. The Burton diphenylamine method and a method based on the indole reaction were found to be the most precise and sensitive of those studied. The Burton method is easy to perform under routine conditions, but the results of analysis are delayed by a lengthy incubation and the reagents require careful purification. Both diphenylamine methods were found to be subject to interference by many substances commonly present in solutions of DNA. The method based on the indole reaction was found to be relatively free from interference. It requires more manipulations than the others, but it is often the best choice when interfering substances are present. A method based on the cysteine reaction showed poor reproducibility under routine use and it proved to be the most troublesome of the four to perform." @default.
• W1982160785 created "2016-06-24" @default.
• W1982160785 creator A5031367754 @default.
• W1982160785 creator A5041438932 @default.
• W1982160785 creator A5063508277 @default.
• W1982160785 date "1968-11-01" @default.
• W1982160785 modified "2023-09-24" @default.
• W1982160785 title "Effect of interfering substances on colorimetric reactions for deoxyribonucleic acid" @default.
• W1982160785 cites W108903188 @default.
• W1982160785 cites W1513897103 @default.
• W1982160785 cites W1581526449 @default.
• W1982160785 cites W1607496744 @default.
• W1982160785 cites W1906197854 @default.
• W1982160785 cites W1988269103 @default.
• W1982160785 cites W203704018 @default.
• W1982160785 cites W2037794097 @default.
• W1982160785 cites W2038124483 @default.
• W1982160785 cites W2048315728 @default.
• W1982160785 cites W2069670696 @default.
• W1982160785 cites W2090937298 @default.
• W1982160785 cites W2417401712 @default.
• W1982160785 cites W94752529 @default.
• W1982160785 doi "https://doi.org/10.1016/0003-2697(68)90336-9" @default.
• W1982160785 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4990828" @default.
• W1982160785 hasPublicationYear "1968" @default.
• W1982160785 type Work @default.
• W1982160785 sameAs 1982160785 @default.
• W1982160785 citedByCount "3" @default.
• W1982160785 crossrefType "journal-article" @default.
• W1982160785 hasAuthorship W1982160785A5031367754 @default.
• W1982160785 hasAuthorship W1982160785A5041438932 @default.
• W1982160785 hasAuthorship W1982160785A5063508277 @default.
• W1982160785 hasConcept C142724271 @default.
• W1982160785 hasConcept C178790620 @default.
• W1982160785 hasConcept C185592680 @default.
• W1982160785 hasConcept C192527728 @default.
• W1982160785 hasConcept C27754629 @default.
• W1982160785 hasConcept C2777040443 @default.
• W1982160785 hasConcept C2780591741 @default.
• W1982160785 hasConcept C2780742167 @default.
• W1982160785 hasConcept C40875361 @default.
• W1982160785 hasConcept C43617362 @default.
• W1982160785 hasConcept C71924100 @default.
• W1982160785 hasConcept C98015330 @default.
• W1982160785 hasConceptScore W1982160785C142724271 @default.
• W1982160785 hasConceptScore W1982160785C178790620 @default.
• W1982160785 hasConceptScore W1982160785C185592680 @default.
• W1982160785 hasConceptScore W1982160785C192527728 @default.
• W1982160785 hasConceptScore W1982160785C27754629 @default.
• W1982160785 hasConceptScore W1982160785C2777040443 @default.
• W1982160785 hasConceptScore W1982160785C2780591741 @default.
• W1982160785 hasConceptScore W1982160785C2780742167 @default.
• W1982160785 hasConceptScore W1982160785C40875361 @default.
• W1982160785 hasConceptScore W1982160785C43617362 @default.
• W1982160785 hasConceptScore W1982160785C71924100 @default.
• W1982160785 hasConceptScore W1982160785C98015330 @default.
• W1982160785 hasIssue "2" @default.
• W1982160785 hasLocation W19821607851 @default.
• W1982160785 hasLocation W19821607852 @default.
• W1982160785 hasOpenAccess W1982160785 @default.
• W1982160785 hasPrimaryLocation W19821607851 @default.
• W1982160785 hasRelatedWork W2098149421 @default.
• W1982160785 hasRelatedWork W2352036717 @default.
• W1982160785 hasRelatedWork W2367381274 @default.
• W1982160785 hasRelatedWork W2367668124 @default.
• W1982160785 hasRelatedWork W2371702910 @default.
• W1982160785 hasRelatedWork W2375439920 @default.
• W1982160785 hasRelatedWork W2383832930 @default.
• W1982160785 hasRelatedWork W2392549443 @default.
• W1982160785 hasRelatedWork W2392617848 @default.
• W1982160785 hasRelatedWork W2393862741 @default.
• W1982160785 hasVolume "26" @default.
• W1982160785 isParatext "false" @default.
• W1982160785 isRetracted "false" @default.
• W1982160785 magId "1982160785" @default.
• W1982160785 workType "article" @default.