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- W1982167029 abstract "The characteristics and advantages of a renewable DNA hybridization biosensor based on a pencil electrode transducer are described. The surface of this biosensor can be renewed rapidly, by a simple mechanical extrusion, hence, obviating the need for an additional regeneration step. The sensor, thus, responds rapidly to the ‘switching’ between target and non-complementary oligonucleotide solutions, with the use of fresh surfaces erasing memory effects. The intrinsic redox activity of the target DNA is employed for detecting the duplex formation. Relevant experimental parameters were examined and optimized. The selectivity of the new device was demonstrated for the detection of a single-point mutation in the BRCA1 breast cancer gene. Such low-cost, renewable graphite transducers provide an attractive alternative to conventional carbon electrodes used for transducing DNA hybridization. By eliminating the needs for a regeneration step and an external indicator, the device offers a greatly simplified operation and holds promise for decentralized genetic testing." @default.
- W1982167029 created "2016-06-24" @default.
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- W1982167029 date "2001-03-01" @default.
- W1982167029 modified "2023-10-15" @default.
- W1982167029 title "Pencil-based renewable biosensor for label-free electrochemical detection of DNA hybridization" @default.
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- W1982167029 doi "https://doi.org/10.1016/s0003-2670(00)01318-0" @default.
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