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- W1982178290 abstract "The effects of humic acid (HA) on the toxicity of copper to sea urchin Paracentrotus lividus larvae were studied in chemically defined seawater. Square Wave Anodic Stripping Voltammetry (SWASV) was employed to study the complexation of copper in seawater medium. A simple complexation model assuming one ligand type and a 1:1 reaction stoichiometry successfully explained the inverse titration experiments. A conditional stability constant of 6.53+/-0.05 and a complexating capacity of 230+/-7 micromol Cu/g HA were obtained. Sea urchin bioassay tests with two endpoints, embryogenesis success and larval growth were carried out in order to study the toxicity of dissolved copper in both the presence and absence of HA. The toxicity data obtained fitted well into a logistic model, and the high sensitivity of both endpoints (EC(50) were 41.1 microg Cu/l and 32.9 microg Cu/l, respectively) encourages their use for biomonitoring. The HA had a clearly protective effect, reducing the toxicity of Cu to the sea urchin larvae. The labile copper, rather than the total copper concentrations, explained the toxicity of the Cu-HA solutions, and the Cu-HA complexes appeared as non-toxic forms. These results are in agreement with the Free Ion Activity Model, because the labile Cu concentrations in this buffered and chemically defined medium covary with the free ion activity of the Cu, validating the model to naturally occurring HA in the marine environment." @default.
- W1982178290 created "2016-06-24" @default.
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- W1982178290 date "2002-07-01" @default.
- W1982178290 modified "2023-10-06" @default.
- W1982178290 title "Effect of humic acids on speciation and toxicity of copper to Paracentrotus lividus larvae in seawater" @default.
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- W1982178290 doi "https://doi.org/10.1016/s0166-445x(01)00219-3" @default.
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