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• W1982244421 endingPage "688" @default.
• W1982244421 startingPage "683" @default.
• W1982244421 abstract "DAX1 is an orphan nuclear receptor and involved in development of steroidogenic organs, which activates transcription of genes involved in steroidogenesis. In this study, we analyzed the function of the zebrafish dax1 during early development of central nervous systems to appear unidentified aspects of DAX1 and decrease confusions concerned with functions of DAX1 in early development of vertebrates. By whole-mount in situ hybridization of embryo at the 32 h post fertilization (hpf), expression of zebrafish dax1 was detected around the forebrain, midbrain, hindbrain, and the extending tail tip. Embryos injected with zebrafish dax1 morpholino antisense nucleotide (MO) exhibited delayed development. When the developmental stage of wild type embryos was at Prim-15 (32 hpf), zebrafish dax1MO injected embryos were at Prim-5 (24 hpf). Concurrently with developmental delay, the MO injected embryos showed high mortality. At 48 hpf, the MO injected embryos exhibited abnormal development in the central nervous systems. The enlarged tectum and the protruded rhombomeres were observed. Moreover, development of central nervous systems, especially midbrain-hindbrain boundary, became narrower. At 5 day post fertilization, the MO injected embryos formed edemas around head, pericardial sac and abdomen. Collectively, our results indicated that the zebrafish dax1 is important for brain development." @default.
• W1982244421 created "2016-06-24" @default.
• W1982244421 creator A5002137179 @default.
• W1982244421 creator A5048189577 @default.
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• W1982244421 date "2012-06-01" @default.
• W1982244421 modified "2023-09-26" @default.
• W1982244421 title "Damage in brain development by morpholino knockdown of zebrafish dax1" @default.
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• W1982244421 doi "https://doi.org/10.1016/j.jbiosc.2012.02.002" @default.
• W1982244421 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22483435" @default.
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