FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1982325535> ?p ?o ?g. }

• W1982325535 endingPage "38" @default.
• W1982325535 startingPage "28" @default.
• W1982325535 abstract "Previous studies have shown that atrial natriuretic peptides inhibit mitogenesis in subcultured aortic smooth muscle cells by a mechanism that appears to be mediated via the C-type or “clearance” receptor. In the current study, we have compared the antimitogenic effect of these peptides in serum-stimulated primary aortic smooth muscle cell cultures and in subcultured cells. A series of atrial peptides, including rANF99–126, rANF103–126, and rANF103–125, were only poorly antimitogenic in serum-stimulated primary cultures, whereas des[Cys105, Cys121] rANF104–126 which binds selectively to the ANF-C receptors had no antimitogenic activity. In contrast, in subcultured cells (between subcultures 3 and 25), rANF99–126, rANF103–126, rANF103–126, Cys116rANF102–116, and des[Cys105, Cys121]rANF104–126 inhibited serum-induced [3H]thymidine incorporation (IC50 in the range of 10–50 nM), with maximal inhibition of 40–70%. The lack of antimitogenic activity in primary cultures did not appear to be related to the lack of cGMP elevation elicited by atrial peptides or to an inherent insensitivity to the action of antimitogens, because primary cultures were responsive to the cGMP-elevating effect of atrial peptides and the cells were more rather than less sensitive to the antimitogenic effect of the nitric-oxide-vasodilator, SNAP, as compared to subcultured cells. Analysis of the affinity and binding capacity of freshly isolated aortic membranes, and primary or secondary cultures for [125I]rANF99–126, revealed that the number of ANF receptors increased by tenfold, following subculture. Moreover, subcultured cells contained receptors with increased binding affinity for peptide analogues selective for the ANF-C-type type receptor. Covalent cross-linking studies with (125I)rANF99–126 confirmed that membranes prepared from fresh aortae predominantly expressed the ANF-A/guanylate cyclase receptor, whereas in subcultured cells the predominantly cross-linked protein was the ANF-C-type receptor, with receptors in primary cultures occupying an intermediate position. These results suggest that the binding and antimitogenic activity of atrial peptides in aortic smooth muscle cells depends on the phenotypic state of these cells. Moreover, the increased antimitogenic potency of atrial peptides in secondary cultures may reflect increased expression of the ANF-C-type receptors. © 1993 Wiley-Liss, Inc." @default.
• W1982325535 created "2016-06-24" @default.
• W1982325535 creator A5019656476 @default.
• W1982325535 creator A5075494047 @default.
• W1982325535 date "1993-01-01" @default.
• W1982325535 modified "2023-09-25" @default.
• W1982325535 title "Differential antimitogenic effectiveness of atrial natriuretic peptides in primary versus subcultured rat aortic smooth muscle cells: Relationship to expression of ANF-C receptors" @default.
• W1982325535 cites W1496216397 @default.
• W1982325535 cites W1524757395 @default.
• W1982325535 cites W1610892577 @default.
• W1982325535 cites W1650161087 @default.
• W1982325535 cites W1775749144 @default.
• W1982325535 cites W1783085374 @default.
• W1982325535 cites W1965279410 @default.
• W1982325535 cites W1967295182 @default.
• W1982325535 cites W1971652814 @default.
• W1982325535 cites W1978404401 @default.
• W1982325535 cites W1980214446 @default.
• W1982325535 cites W1999815931 @default.
• W1982325535 cites W2010249501 @default.
• W1982325535 cites W2011288528 @default.
• W1982325535 cites W2016368344 @default.
• W1982325535 cites W2018142079 @default.
• W1982325535 cites W2041075406 @default.
• W1982325535 cites W2043813981 @default.
• W1982325535 cites W2049520832 @default.
• W1982325535 cites W2050961361 @default.
• W1982325535 cites W2074052803 @default.
• W1982325535 cites W2078349803 @default.
• W1982325535 cites W2083647583 @default.
• W1982325535 cites W2098507108 @default.
• W1982325535 cites W2119455028 @default.
• W1982325535 cites W2146087296 @default.
• W1982325535 cites W2154616804 @default.
• W1982325535 cites W2285606889 @default.
• W1982325535 cites W2415535951 @default.
• W1982325535 cites W2418260579 @default.
• W1982325535 cites W2044583677 @default.
• W1982325535 doi "https://doi.org/10.1002/jcp.1041540105" @default.
• W1982325535 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7678266" @default.
• W1982325535 hasPublicationYear "1993" @default.
• W1982325535 type Work @default.
• W1982325535 sameAs 1982325535 @default.
• W1982325535 citedByCount "32" @default.
• W1982325535 countsByYear W19823255352014 @default.
• W1982325535 countsByYear W19823255352016 @default.
• W1982325535 countsByYear W19823255352018 @default.
• W1982325535 crossrefType "journal-article" @default.
• W1982325535 hasAuthorship W1982325535A5019656476 @default.
• W1982325535 hasAuthorship W1982325535A5075494047 @default.
• W1982325535 hasConcept C126322002 @default.
• W1982325535 hasConcept C134018914 @default.
• W1982325535 hasConcept C153911025 @default.
• W1982325535 hasConcept C170493617 @default.
• W1982325535 hasConcept C185592680 @default.
• W1982325535 hasConcept C202751555 @default.
• W1982325535 hasConcept C2775915353 @default.
• W1982325535 hasConcept C2778069365 @default.
• W1982325535 hasConcept C2778198053 @default.
• W1982325535 hasConcept C2779281246 @default.
• W1982325535 hasConcept C2779395532 @default.
• W1982325535 hasConcept C2992686903 @default.
• W1982325535 hasConcept C55493867 @default.
• W1982325535 hasConcept C59822182 @default.
• W1982325535 hasConcept C71924100 @default.
• W1982325535 hasConcept C86803240 @default.
• W1982325535 hasConcept C87863119 @default.
• W1982325535 hasConceptScore W1982325535C126322002 @default.
• W1982325535 hasConceptScore W1982325535C134018914 @default.
• W1982325535 hasConceptScore W1982325535C153911025 @default.
• W1982325535 hasConceptScore W1982325535C170493617 @default.
• W1982325535 hasConceptScore W1982325535C185592680 @default.
• W1982325535 hasConceptScore W1982325535C202751555 @default.
• W1982325535 hasConceptScore W1982325535C2775915353 @default.
• W1982325535 hasConceptScore W1982325535C2778069365 @default.
• W1982325535 hasConceptScore W1982325535C2778198053 @default.
• W1982325535 hasConceptScore W1982325535C2779281246 @default.
• W1982325535 hasConceptScore W1982325535C2779395532 @default.
• W1982325535 hasConceptScore W1982325535C2992686903 @default.
• W1982325535 hasConceptScore W1982325535C55493867 @default.
• W1982325535 hasConceptScore W1982325535C59822182 @default.
• W1982325535 hasConceptScore W1982325535C71924100 @default.
• W1982325535 hasConceptScore W1982325535C86803240 @default.
• W1982325535 hasConceptScore W1982325535C87863119 @default.
• W1982325535 hasIssue "1" @default.
• W1982325535 hasLocation W19823255351 @default.
• W1982325535 hasLocation W19823255352 @default.
• W1982325535 hasOpenAccess W1982325535 @default.
• W1982325535 hasPrimaryLocation W19823255351 @default.
• W1982325535 hasRelatedWork W1970446704 @default.
• W1982325535 hasRelatedWork W1971613811 @default.
• W1982325535 hasRelatedWork W2002038679 @default.
• W1982325535 hasRelatedWork W2043879443 @default.
• W1982325535 hasRelatedWork W2049832880 @default.
• W1982325535 hasRelatedWork W2098870266 @default.
• W1982325535 hasRelatedWork W2131191938 @default.
• W1982325535 hasRelatedWork W2153348972 @default.
• W1982325535 hasRelatedWork W2161501502 @default.

• next