FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1982364113> ?p ?o ?g. }

• W1982364113 endingPage "9" @default.
• W1982364113 startingPage "1" @default.
• W1982364113 abstract "Abstract Endothelial cells synthesize and secrete urokinase-type plasminogen activators (u-PA) in response to various stimuli. To modulate the local expression of u-PA activity both intravascularly and pericellularly during angiogenesis, endothelial cells express both inhibitors (primarily PAI-1) and receptors (u-PAR) for u-PA. The interaction of u-PA with receptors on the surface of endothelial cells may play an important role in the regulation of fibrinolysis and cell migration. Using radioligand binding studies, we and others have demonstrated that human umbilical vein endothelial cells (HUVEC) express high affinity receptors for urokinase on the cell surface. We have demonstrated that single chain urokinase (scu-PA, prourokinase) binds only via the growth factor domain, while two chain high molecular weight urokinase (tcu-PA) can bind to the receptor or to cell- or matrix-associated PAI-1. We have isolated a ∼46kDa glycoprotein from HUVEC using affinity chromatography which retains the ability to specifically bind u-PA. At least three post -translational modifications appear to occur including removal of an N-terminal signal peptide; N-linked glycosylation, and C-terminal cleavage with addition of a phosphatidylinositol-glycan moiety which links the externally oriented protein to the cell surface. Using the polymerase chain reaction and published sequence information of the u-PAR cloned from a transformed fibroblast cDNA library, we amplified cDNAs of u-PAR from HUVEC and PMA-treated U937 cells. The specificity of the cDNAs was confirmed by restriction mapping and direct sequence analysis. Using these probes and radioligand binding studies we have demonstrated that at least two independent protein kinase pathways exist in endothelial cells for upregulating u-PA receptor expression. Down regulation of receptors may be pathophysiologic in thrombosic disorders whereas up regulation may be important in promoting wound healing, vascular repair, and protection from thrombosis. Up regulation could be harmful as well in such conditions as pathologic neovascularization (e.g., diabetic retinopathy) and in tumour metastasis as well as in tumour-related angiogenesis. Understanding the control and functional significance of u-PA binding to cells in general will hopefully enable the design of therapies to optimize the beneficial aspects and minimize any harmful effects of this interaction. Changes in the local expression of u-PA, PAM and u-PAR by endothelial cells may affect the extent of angiogenesis or the degree of local intravascular fibrinolysis, which might be critical in conditions such as unstable angina and myocardial infarction." @default.
• W1982364113 created "2016-06-24" @default.
• W1982364113 creator A5003992000 @default.
• W1982364113 date "1992-01-01" @default.
• W1982364113 modified "2023-10-14" @default.
• W1982364113 title "Characterization and regulation of the urokinase receptor of human endothelial cells" @default.
• W1982364113 cites W1487780734 @default.
• W1982364113 cites W1495239533 @default.
• W1982364113 cites W1497832606 @default.
• W1982364113 cites W1513184466 @default.
• W1982364113 cites W1537552785 @default.
• W1982364113 cites W1548496378 @default.
• W1982364113 cites W1549257577 @default.
• W1982364113 cites W1558212310 @default.
• W1982364113 cites W1559877912 @default.
• W1982364113 cites W1566704447 @default.
• W1982364113 cites W1595391532 @default.
• W1982364113 cites W1605654974 @default.
• W1982364113 cites W1611413521 @default.
• W1982364113 cites W1618760884 @default.
• W1982364113 cites W1853643611 @default.
• W1982364113 cites W1963621811 @default.
• W1982364113 cites W1971906639 @default.
• W1982364113 cites W1999757767 @default.
• W1982364113 cites W2001124978 @default.
• W1982364113 cites W2001704825 @default.
• W1982364113 cites W2008980811 @default.
• W1982364113 cites W2011936885 @default.
• W1982364113 cites W2014086967 @default.
• W1982364113 cites W2014664823 @default.
• W1982364113 cites W2029858462 @default.
• W1982364113 cites W2031248346 @default.
• W1982364113 cites W2042314261 @default.
• W1982364113 cites W2046787082 @default.
• W1982364113 cites W2047369161 @default.
• W1982364113 cites W2047797817 @default.
• W1982364113 cites W2048214708 @default.
• W1982364113 cites W2049631846 @default.
• W1982364113 cites W2049661577 @default.
• W1982364113 cites W2052700106 @default.
• W1982364113 cites W2053033421 @default.
• W1982364113 cites W2056274413 @default.
• W1982364113 cites W2057056866 @default.
• W1982364113 cites W2069289312 @default.
• W1982364113 cites W2073748704 @default.
• W1982364113 cites W2075233240 @default.
• W1982364113 cites W2079568721 @default.
• W1982364113 cites W2087375731 @default.
• W1982364113 cites W2087767229 @default.
• W1982364113 cites W2098335293 @default.
• W1982364113 cites W2100918981 @default.
• W1982364113 cites W2101487931 @default.
• W1982364113 cites W2101902674 @default.
• W1982364113 cites W2103689548 @default.
• W1982364113 cites W2106933686 @default.
• W1982364113 cites W2109320108 @default.
• W1982364113 cites W2120202594 @default.
• W1982364113 cites W2120839048 @default.
• W1982364113 cites W2134311390 @default.
• W1982364113 cites W2142633589 @default.
• W1982364113 cites W2145246787 @default.
• W1982364113 cites W2158431724 @default.
• W1982364113 cites W2170260797 @default.
• W1982364113 cites W2247637534 @default.
• W1982364113 cites W23122061 @default.
• W1982364113 cites W2406575963 @default.
• W1982364113 cites W2408590235 @default.
• W1982364113 cites W2412703172 @default.
• W1982364113 cites W2415457672 @default.
• W1982364113 cites W2419316371 @default.
• W1982364113 cites W4214580768 @default.
• W1982364113 cites W4244633958 @default.
• W1982364113 cites W45293395 @default.
• W1982364113 doi "https://doi.org/10.1016/0268-9499(92)90086-w" @default.
• W1982364113 hasPublicationYear "1992" @default.
• W1982364113 type Work @default.
• W1982364113 sameAs 1982364113 @default.
• W1982364113 citedByCount "13" @default.
• W1982364113 crossrefType "journal-article" @default.
• W1982364113 hasAuthorship W1982364113A5003992000 @default.
• W1982364113 hasBestOaLocation W19823641131 @default.
• W1982364113 hasConcept C170493617 @default.
• W1982364113 hasConcept C185592680 @default.
• W1982364113 hasConcept C2776892390 @default.
• W1982364113 hasConcept C54355233 @default.
• W1982364113 hasConcept C55493867 @default.
• W1982364113 hasConcept C56219504 @default.
• W1982364113 hasConcept C86803240 @default.
• W1982364113 hasConcept C95444343 @default.
• W1982364113 hasConceptScore W1982364113C170493617 @default.
• W1982364113 hasConceptScore W1982364113C185592680 @default.
• W1982364113 hasConceptScore W1982364113C2776892390 @default.
• W1982364113 hasConceptScore W1982364113C54355233 @default.
• W1982364113 hasConceptScore W1982364113C55493867 @default.
• W1982364113 hasConceptScore W1982364113C56219504 @default.
• W1982364113 hasConceptScore W1982364113C86803240 @default.
• W1982364113 hasConceptScore W1982364113C95444343 @default.
• W1982364113 hasLocation W19823641131 @default.

• next