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- W1982380137 abstract "A method for flow-through liquid scintillation counting in reversed-phase liquid chromatography (RP-LC) based on the continuous extraction of aqueous column eluates with a water-immiscible liquid scintillator is evaluated in terms of sensitivity, reproducibility and extra-column band broadening. 3H- and 14C-labelled phenylthiohydantoinamino acid derivatives of widely different polarity serve as model compounds. For extractable derivatives, counting efficiencies of over 30 and 80% can be obtained for 3H and 14C, respectively. The reproducibility and extra-column band broadening depend on the mixing ratio of scintillator and LC eluent; relative standard deviations (peak areas) of less than 3% can be obtained. The sensitivity of flow-through counting can be increased at least 150-fold by storing the segmented scintillator/eluate stream in a capillary storage loop. After the separation is complete, the stream is re-introduced into the radioactivity detector at reduced flow-rates to increase the mean residence time, i.e., the counting time, in the detector." @default.
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- W1982380137 date "1985-01-01" @default.
- W1982380137 modified "2023-10-16" @default.
- W1982380137 title "High-performance liquid chromatographic support for the baseline separation of all phenylthiohydantoin amino acids" @default.
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- W1982380137 doi "https://doi.org/10.1016/s0378-4347(00)84530-2" @default.
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