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- W1982393669 endingPage "e0121830" @default.
- W1982393669 startingPage "e0121830" @default.
- W1982393669 abstract "Alanine aminotransferase (AlaAT, E.C. 2.6.1.2), is a pyridoxal-5’-phosphate-dependent (PLP) enzyme that catalyzes the reversible transfer of an amino group from alanine to 2-oxoglutarate to produce glutamate and pyruvate, or vice versa. It has been well documented in both greenhouse and field studies that tissue-specific over-expression of AlaAT from barley (Hordeum vulgare, HvAlaAT) results in a significant increase in plant NUE in both canola and rice. While the physical phenotypes associated with over-expression of HvAlaAT have been well characterized, the role this enzyme plays in vivo to create a more N efficient plant remains unknown. Furthermore, the importance of HvAlaAT, in contrast to other AlaAT enzyme homologues in creating this phenotype has not yet been explored. To address the role of AlaAT in NUE, AlaAT variants from diverse sources and different subcellular locations, were expressed in the wild-type Arabidopsis thaliana Col-0 background and alaat1;2 (alaat1-1;alaat2-1) knockout background in various N environments. The analysis and comparison of both the physical and physiological properties of AlaAT over-expressing transgenic plants demonstrated significant differences between plants expressing the different AlaAT enzymes under different external conditions. This analysis indicates that the over-expression of AlaAT variants other than HvAlaAT in crop plants could further increase the NUE phenotype(s) previously observed." @default.
- W1982393669 created "2016-06-24" @default.
- W1982393669 creator A5023270263 @default.
- W1982393669 creator A5085988251 @default.
- W1982393669 date "2015-04-01" @default.
- W1982393669 modified "2023-10-11" @default.
- W1982393669 title "Alanine Aminotransferase Variants Conferring Diverse NUE Phenotypes in Arabidopsis thaliana" @default.
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- W1982393669 doi "https://doi.org/10.1371/journal.pone.0121830" @default.
- W1982393669 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4382294" @default.
- W1982393669 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25830496" @default.
- W1982393669 hasPublicationYear "2015" @default.
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