Matches in SemOpenAlex for { <https://semopenalex.org/work/W1982397424> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W1982397424 endingPage "889" @default.
- W1982397424 startingPage "881" @default.
- W1982397424 abstract "Cyanovirin-N (CVN) is a novel cyanobacterial protein that selectively binds with nanomolar affinities the mammalian oligosaccharides Man(8) and Man(9). Consequently, CVN potently blocks HIV entry through highly avid carbohydrate-mediated interactions with the HIV-envelope glycoprotein gp120, and is under preclinical investigation as an anti-HIV microbicide. CVN contains two non-overlapping carbohydrate-binding sites that bind the disaccharide Manalpha(1-2)Manalpha (which represents the terminal disaccharide of all three arms of Man(9)) with low to sub-micromolar affinities. The solution structure of a 1:2 CVN:Manalpha(1-2)Manalpha complex revealed that CVN recognizes the stacked conformation of Manalpha(1-2)Manalpha through a deep hydrophilic-binding pocket on one side of the protein (site 2) and a semi-circular cleft on the other (site 1). With the prominent exception of the C1 hydroxyl group of the reducing mannopyranose ring, the bound disaccharide is positioned so that each hydroxyl group is involved in a direct or water-mediated hydrogen bond to the polar or charged side-chains comprising the binding pocket. Thus, to determine whether the next-most reducing mannopyranose ring will augment CVN affinity and selectivity, we have characterized by NMR and ITC the binding of CVN to three synthetic trisaccharides representing the full-length D1, D2 and D3 arms of mammalian oligomannosides. Our findings demonstrate that site 1 is able to discriminate between the three related trisaccharides methyl Manalpha(1-2)Manalpha(1-2)Man, methyl Manalpha(1-2)Manalpha(1-3)Man and methyl Manalpha(1-2)Manalpha(1-6)Man with remarkable selectivity, and binds these trisaccharides with K(A) values ranging from 8.1x10(3)M(-1) to 6.6x10(6)M(-1). Site 2 is less selective in that it binds all three trisaccharides with similar K(A) values ranging from 1.7 to 3.7(+/-0.3)x10(5)M(-1), but overall binds these trimannosides with higher affinities than site 1. The diversity of pathogenic organisms that display alpha(1-2)-linked mannosides on their cell surfaces suggests a broad defensive role for CVN in its cyanobacterial source." @default.
- W1982397424 created "2016-06-24" @default.
- W1982397424 creator A5021730310 @default.
- W1982397424 creator A5046015612 @default.
- W1982397424 creator A5091606860 @default.
- W1982397424 date "2002-09-01" @default.
- W1982397424 modified "2023-09-23" @default.
- W1982397424 title "Site-specific Discrimination by Cyanovirin-N for α-Linked Trisaccharides Comprising the Three Arms of Man8 and Man9" @default.
- W1982397424 cites W1811324376 @default.
- W1982397424 cites W1967703272 @default.
- W1982397424 cites W1970108540 @default.
- W1982397424 cites W1979736443 @default.
- W1982397424 cites W2002662064 @default.
- W1982397424 cites W2013937866 @default.
- W1982397424 cites W2062148542 @default.
- W1982397424 cites W2076595001 @default.
- W1982397424 cites W2083381419 @default.
- W1982397424 cites W2093709579 @default.
- W1982397424 cites W2138802639 @default.
- W1982397424 cites W2143212326 @default.
- W1982397424 cites W2169821755 @default.
- W1982397424 cites W2170748261 @default.
- W1982397424 cites W2416039323 @default.
- W1982397424 cites W2951215598 @default.
- W1982397424 cites W2951997476 @default.
- W1982397424 cites W4251775387 @default.
- W1982397424 cites W4317929392 @default.
- W1982397424 doi "https://doi.org/10.1016/s0022-2836(02)00842-2" @default.
- W1982397424 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12270721" @default.
- W1982397424 hasPublicationYear "2002" @default.
- W1982397424 type Work @default.
- W1982397424 sameAs 1982397424 @default.
- W1982397424 citedByCount "66" @default.
- W1982397424 countsByYear W19823974242012 @default.
- W1982397424 countsByYear W19823974242013 @default.
- W1982397424 countsByYear W19823974242014 @default.
- W1982397424 countsByYear W19823974242015 @default.
- W1982397424 countsByYear W19823974242017 @default.
- W1982397424 countsByYear W19823974242018 @default.
- W1982397424 countsByYear W19823974242019 @default.
- W1982397424 countsByYear W19823974242020 @default.
- W1982397424 countsByYear W19823974242021 @default.
- W1982397424 countsByYear W19823974242022 @default.
- W1982397424 countsByYear W19823974242023 @default.
- W1982397424 crossrefType "journal-article" @default.
- W1982397424 hasAuthorship W1982397424A5021730310 @default.
- W1982397424 hasAuthorship W1982397424A5046015612 @default.
- W1982397424 hasAuthorship W1982397424A5091606860 @default.
- W1982397424 hasConcept C107824862 @default.
- W1982397424 hasConcept C112887158 @default.
- W1982397424 hasConcept C170493617 @default.
- W1982397424 hasConcept C178790620 @default.
- W1982397424 hasConcept C185592680 @default.
- W1982397424 hasConcept C2777346074 @default.
- W1982397424 hasConcept C2780283098 @default.
- W1982397424 hasConcept C3018795828 @default.
- W1982397424 hasConcept C32909587 @default.
- W1982397424 hasConcept C55493867 @default.
- W1982397424 hasConcept C71240020 @default.
- W1982397424 hasConceptScore W1982397424C107824862 @default.
- W1982397424 hasConceptScore W1982397424C112887158 @default.
- W1982397424 hasConceptScore W1982397424C170493617 @default.
- W1982397424 hasConceptScore W1982397424C178790620 @default.
- W1982397424 hasConceptScore W1982397424C185592680 @default.
- W1982397424 hasConceptScore W1982397424C2777346074 @default.
- W1982397424 hasConceptScore W1982397424C2780283098 @default.
- W1982397424 hasConceptScore W1982397424C3018795828 @default.
- W1982397424 hasConceptScore W1982397424C32909587 @default.
- W1982397424 hasConceptScore W1982397424C55493867 @default.
- W1982397424 hasConceptScore W1982397424C71240020 @default.
- W1982397424 hasIssue "4" @default.
- W1982397424 hasLocation W19823974241 @default.
- W1982397424 hasLocation W19823974242 @default.
- W1982397424 hasOpenAccess W1982397424 @default.
- W1982397424 hasPrimaryLocation W19823974241 @default.
- W1982397424 hasRelatedWork W1933643218 @default.
- W1982397424 hasRelatedWork W1982397424 @default.
- W1982397424 hasRelatedWork W1996743374 @default.
- W1982397424 hasRelatedWork W2002662064 @default.
- W1982397424 hasRelatedWork W2042990078 @default.
- W1982397424 hasRelatedWork W2052512234 @default.
- W1982397424 hasRelatedWork W2070368353 @default.
- W1982397424 hasRelatedWork W2949759959 @default.
- W1982397424 hasRelatedWork W2953257930 @default.
- W1982397424 hasRelatedWork W4252791512 @default.
- W1982397424 hasVolume "322" @default.
- W1982397424 isParatext "false" @default.
- W1982397424 isRetracted "false" @default.
- W1982397424 magId "1982397424" @default.
- W1982397424 workType "article" @default.