Matches in SemOpenAlex for { <https://semopenalex.org/work/W1982485428> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W1982485428 endingPage "274" @default.
- W1982485428 startingPage "268" @default.
- W1982485428 abstract "The occluded form of baculoviruses is much less infectious to cell cultures than the extracellular virus (ECV). In studies using alkaline solutions or insect gut fluids, from 3,600 to 54,000 occlusion bodies (OBs) were needed per infectious unit. Yet there are reasons for wanting to use OBs, namely, for monitoring the efficacy of polyhedra and to avoid problems associated with long-term passage of the ECV. In the present study, gypsy moth nuclear polyhedrosis virus OBs were dissolved in a sodium bicarbonate/sodium chloride solution and used to infect the gypsy moth fat body cell line (IPLB-LdFBc1). Using 27 mM Na2CO3 and 54 mM NaCl, the optimum time of dissolution was 30 min and approximately 1200 OBs were necessary to obtain one infectious unit. In addition, treatment of the dissolved OBs with 90 μg/ml trypsin for 2 hr resulted in only 21 OBs being necessary to obtain one infectious unit, an improvement of over 50-fold. Also, comparison with other cell lines showed two embryonic cell lines (IPLB-LdEp and IPLB-LdEIta) to be more susceptible to dissolved OBs. However, the infectivity to these other cell lines was not improved by trypsinization of dissolved OBs. Infection of fat body cell cultures through multiple passages using dissolved and trypsinized OBs still resulted in a decline in OB productivity over time but to a lesser degree than that with ECV. The same studies showed no increase in few polyhedra (FP) mutants when the virus was passed 15 times using polyhedra-derived virus, compared with an approximately 20% increase in FP mutants when ECV was used." @default.
- W1982485428 created "2016-06-24" @default.
- W1982485428 creator A5059868158 @default.
- W1982485428 date "1994-05-01" @default.
- W1982485428 modified "2023-09-23" @default.
- W1982485428 title "Enhanced Infectivity of Occluded Virions of the Gypsy Moth Nuclear Polyhedrosis Virus for Cell Cultures" @default.
- W1982485428 doi "https://doi.org/10.1006/jipa.1994.1051" @default.
- W1982485428 hasPublicationYear "1994" @default.
- W1982485428 type Work @default.
- W1982485428 sameAs 1982485428 @default.
- W1982485428 citedByCount "18" @default.
- W1982485428 countsByYear W19824854282012 @default.
- W1982485428 countsByYear W19824854282014 @default.
- W1982485428 countsByYear W19824854282016 @default.
- W1982485428 countsByYear W19824854282023 @default.
- W1982485428 crossrefType "journal-article" @default.
- W1982485428 hasAuthorship W1982485428A5059868158 @default.
- W1982485428 hasConcept C104317684 @default.
- W1982485428 hasConcept C106358424 @default.
- W1982485428 hasConcept C139149728 @default.
- W1982485428 hasConcept C147789679 @default.
- W1982485428 hasConcept C159047783 @default.
- W1982485428 hasConcept C178790620 @default.
- W1982485428 hasConcept C181199279 @default.
- W1982485428 hasConcept C185592680 @default.
- W1982485428 hasConcept C2522874641 @default.
- W1982485428 hasConcept C2777212814 @default.
- W1982485428 hasConcept C2777773057 @default.
- W1982485428 hasConcept C2779665282 @default.
- W1982485428 hasConcept C2780340462 @default.
- W1982485428 hasConcept C38240268 @default.
- W1982485428 hasConcept C40767141 @default.
- W1982485428 hasConcept C537181965 @default.
- W1982485428 hasConcept C54355233 @default.
- W1982485428 hasConcept C55493867 @default.
- W1982485428 hasConcept C81885089 @default.
- W1982485428 hasConcept C86803240 @default.
- W1982485428 hasConceptScore W1982485428C104317684 @default.
- W1982485428 hasConceptScore W1982485428C106358424 @default.
- W1982485428 hasConceptScore W1982485428C139149728 @default.
- W1982485428 hasConceptScore W1982485428C147789679 @default.
- W1982485428 hasConceptScore W1982485428C159047783 @default.
- W1982485428 hasConceptScore W1982485428C178790620 @default.
- W1982485428 hasConceptScore W1982485428C181199279 @default.
- W1982485428 hasConceptScore W1982485428C185592680 @default.
- W1982485428 hasConceptScore W1982485428C2522874641 @default.
- W1982485428 hasConceptScore W1982485428C2777212814 @default.
- W1982485428 hasConceptScore W1982485428C2777773057 @default.
- W1982485428 hasConceptScore W1982485428C2779665282 @default.
- W1982485428 hasConceptScore W1982485428C2780340462 @default.
- W1982485428 hasConceptScore W1982485428C38240268 @default.
- W1982485428 hasConceptScore W1982485428C40767141 @default.
- W1982485428 hasConceptScore W1982485428C537181965 @default.
- W1982485428 hasConceptScore W1982485428C54355233 @default.
- W1982485428 hasConceptScore W1982485428C55493867 @default.
- W1982485428 hasConceptScore W1982485428C81885089 @default.
- W1982485428 hasConceptScore W1982485428C86803240 @default.
- W1982485428 hasIssue "3" @default.
- W1982485428 hasLocation W19824854281 @default.
- W1982485428 hasOpenAccess W1982485428 @default.
- W1982485428 hasPrimaryLocation W19824854281 @default.
- W1982485428 hasRelatedWork W1544310549 @default.
- W1982485428 hasRelatedWork W1897057051 @default.
- W1982485428 hasRelatedWork W1975077568 @default.
- W1982485428 hasRelatedWork W1984887955 @default.
- W1982485428 hasRelatedWork W1987996073 @default.
- W1982485428 hasRelatedWork W1994535548 @default.
- W1982485428 hasRelatedWork W2020475514 @default.
- W1982485428 hasRelatedWork W2093644807 @default.
- W1982485428 hasRelatedWork W2156562827 @default.
- W1982485428 hasRelatedWork W2160389669 @default.
- W1982485428 hasVolume "63" @default.
- W1982485428 isParatext "false" @default.
- W1982485428 isRetracted "false" @default.
- W1982485428 magId "1982485428" @default.
- W1982485428 workType "article" @default.