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• W1982485959 abstract "SUMMARY The pathway for copper (Cu) uptake across the mucosal membrane into intestinal cells has not been elucidated in fish. Copper accumulation in freshly isolated intestinal cells from rainbow trout Oncorhynchus mykiss was measured after exposure to 0–800 μmol l–1 CuSO4 for 15 min. With external Cu concentration (Cuo) of 800 μmol l–1, the rate of Cu accumulation by cells was 1.88±0.52 nmol Cu mg–1cell protein h–1 compared to 0.05±0.01 nmol Cu mg–1 cell protein h–1 with no added Cuo (means ± s.e.m., N=6). Deduction of a rapid Cu accumulation measured on/in cells at time zero (about 12% of the total Cu uptake when Cuo was 800 μmol l–1)revealed a saturable uptake curve, which reached a plateau at 400 μmol l–1 Cuo (Km=216 μmol l–1 Cuo; Vmax=1.09 nmol Cu mg–1 cell protein h–1; 140 mmol l–1 NaCl throughout). Incubation of cells at 4°C did not prevent Cu accumulation. Lowering external [Na+] to 11 mmol l–1 (low Na+o) generally did not alter the rate of Cu accumulation into the cells over a 15 min period. Under low Na+o conditions Cu accumulation was exponential(non-saturable). Na+-insensitive Cu accumulation dominated (59% of total Cu accumulation) when Cuo was 400 μmol l–1 or less. At high Cuo (800 μmol l–1), removal of Na+ caused a 45% increase in Cu accumulation. Pre-incubation of cells with blocking agents of epithelial Na+ channel (ENaC) for 15 min (normal [NaCl] throughout) caused Cu accumulation rates to increase by 40-fold (100 μmol l–1phenamil), 21-fold (10 μmol l–1 CDPC) or 12-fold (2 mmol l–1 amiloride) when Cuo was 800 μmol l–1 compared to those in drug-free controls. Lowering the external chloride concentration [Cl–]o from 131.6 to 6.6 mmol l–1 (replaced by sodium gluconate) caused the rate of Cu accumulation to increase 11-fold when Cuo was 800μmol l–1. Application of 0.1 mmol l–1DIDS (normal Cl–o) caused a similar effect. Lowering external pH from 7.4 to pH 5.5 produced a 17-fold, saturable,increase in Cu accumulation rate, which was not explained by increased instantaneous Cu accumulation on/in cells at low pH. We conclude that Cu accumulation by intestinal cells is mainly Na+-insensitive and more characteristic of a pH- and K+-sensitive Ctr1-like pathway than Cu uptake through ENaCs." @default.
• W1982485959 created "2016-06-24" @default.
• W1982485959 creator A5041196638 @default.
• W1982485959 creator A5085099302 @default.
• W1982485959 date "2005-01-15" @default.
• W1982485959 modified "2023-09-27" @default.
• W1982485959 title "Sodium-sensitive and -insensitive copper accumulation by isolated intestinal cells of rainbow trout<i>Oncorhynchus mykiss</i>" @default.
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• W1982485959 cites W1568628619 @default.
• W1982485959 cites W1582077381 @default.
• W1982485959 cites W1768015712 @default.
• W1982485959 cites W1869259273 @default.
• W1982485959 cites W1921402995 @default.
• W1982485959 cites W1926943071 @default.
• W1982485959 cites W1964723543 @default.
• W1982485959 cites W1969904696 @default.
• W1982485959 cites W1972262129 @default.
• W1982485959 cites W1975645479 @default.
• W1982485959 cites W1975758347 @default.
• W1982485959 cites W1980551228 @default.
• W1982485959 cites W1984712233 @default.
• W1982485959 cites W1986238532 @default.
• W1982485959 cites W1986983479 @default.
• W1982485959 cites W1991273560 @default.
• W1982485959 cites W1991274408 @default.
• W1982485959 cites W1991369674 @default.
• W1982485959 cites W1999389252 @default.
• W1982485959 cites W2012250035 @default.
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• W1982485959 cites W2030756048 @default.
• W1982485959 cites W2032457764 @default.
• W1982485959 cites W2038342202 @default.
• W1982485959 cites W2041083875 @default.
• W1982485959 cites W2042099418 @default.
• W1982485959 cites W2049942220 @default.
• W1982485959 cites W2053746701 @default.
• W1982485959 cites W2055765138 @default.
• W1982485959 cites W2062019245 @default.
• W1982485959 cites W2063157862 @default.
• W1982485959 cites W2063207694 @default.
• W1982485959 cites W2064874854 @default.
• W1982485959 cites W2075402276 @default.
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• W1982485959 cites W2091025692 @default.
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• W1982485959 cites W2105193668 @default.
• W1982485959 cites W2105279904 @default.
• W1982485959 cites W2106392025 @default.
• W1982485959 cites W2107985687 @default.
• W1982485959 cites W2111712036 @default.
• W1982485959 cites W2113075361 @default.
• W1982485959 cites W2115724490 @default.
• W1982485959 cites W2117869728 @default.
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• W1982485959 cites W2122190420 @default.
• W1982485959 cites W2129520922 @default.
• W1982485959 cites W2132285093 @default.
• W1982485959 cites W2134169695 @default.
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• W1982485959 doi "https://doi.org/10.1242/jeb.01379" @default.
• W1982485959 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15634857" @default.
• W1982485959 hasPublicationYear "2005" @default.
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