FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1982506386> ?p ?o ?g. }

• W1982506386 endingPage "12290" @default.
• W1982506386 startingPage "12275" @default.
• W1982506386 abstract "The first synthesis of the new C-branched spermine derivative 17, as well as its ability to stabilise DNA duplexes and triplexes, are reported. The C-branched spermine block 17 was converted into the corresponding O-(2-cyanoethyl)-(N,N-diisopropyl)phosphoramidite block 18 for incorporation at the 5′-end of DNA. It was also coupled to the 2′ of ara-U through a phosphate bridge, leading to the partially protected 3′-hydroxy block 23, which was either converted to the O-(2-cyanoethyl)-(N,N-diisopropyl)phosphoramidite24 or to the 3′-succinate block 25, leading to the synthesis of three model DNA 14-mers: 27 with tethered spermine at the 5′-end, 28 with tethered spermine at the middle of the DNA strand, and 29 with spermine at the 2′-end, using standard automated solid-phase chemistry and deprotection procedures. The Tm measurements showed that, at low salt with Mg2+ within the pH range of 5.5 – 7.6, the 5′-spermine-DNA conjugate 27 gives stabilised DNA triplexes with ΔhTms of ∼+3° C and ΔcTms of +5.5° to +8.5° C and the 2′-spermine-DNA conjugate 29 gives ΔhTms of ∼+5° to +6.5° C and ΔcTms of +7.5° to +9° C over the underivatised DNA counterpart 30. In contrast, the spermine block conjugated to the middle of the DNA as in 28 gave no triplex formation. Without Mg2+, these short T-rich oligonucleotides (27, 29 & 30) gave triplex formation only above 0.4M NaCl. At 1.4M, the ΔhTms for 27 and 29 over 30 were +9.5° C while the ΔcTms were +6° to +7° C. Oligonucleotide conjugates 27 & 29 gave only weak duplex stabilisations with ΔhTms of ≈+2° and ≈+1° C, respectively, over 30 at low salt with Mg2+ within the pH range 5.5 – 7.6." @default.
• W1982506386 created "2016-06-24" @default.
• W1982506386 creator A5058088923 @default.
• W1982506386 creator A5068711541 @default.
• W1982506386 creator A5078187729 @default.
• W1982506386 date "1996-09-01" @default.
• W1982506386 modified "2023-10-09" @default.
• W1982506386 title "Synthesis of C-branched spermine tethered oligo-DNA and the thermal stability of the duplexes and triplexes" @default.
• W1982506386 cites W1964658576 @default.
• W1982506386 cites W1966347067 @default.
• W1982506386 cites W1970429992 @default.
• W1982506386 cites W1972570207 @default.
• W1982506386 cites W1985096669 @default.
• W1982506386 cites W1988844434 @default.
• W1982506386 cites W1993996855 @default.
• W1982506386 cites W2000707502 @default.
• W1982506386 cites W2009296586 @default.
• W1982506386 cites W2012959241 @default.
• W1982506386 cites W2018698478 @default.
• W1982506386 cites W2020048556 @default.
• W1982506386 cites W2025114376 @default.
• W1982506386 cites W2030330503 @default.
• W1982506386 cites W2036985289 @default.
• W1982506386 cites W2037987922 @default.
• W1982506386 cites W2044395210 @default.
• W1982506386 cites W2046465265 @default.
• W1982506386 cites W2047282459 @default.
• W1982506386 cites W2052592587 @default.
• W1982506386 cites W2062782594 @default.
• W1982506386 cites W2066124888 @default.
• W1982506386 cites W2067816361 @default.
• W1982506386 cites W2090353848 @default.
• W1982506386 cites W2092975717 @default.
• W1982506386 cites W2094909260 @default.
• W1982506386 cites W2110978538 @default.
• W1982506386 cites W2122117524 @default.
• W1982506386 cites W2132509864 @default.
• W1982506386 cites W2190330093 @default.
• W1982506386 cites W2952397430 @default.
• W1982506386 cites W981578698 @default.
• W1982506386 doi "https://doi.org/10.1016/0040-4020(96)00731-4" @default.
• W1982506386 hasPublicationYear "1996" @default.
• W1982506386 type Work @default.
• W1982506386 sameAs 1982506386 @default.
• W1982506386 citedByCount "35" @default.
• W1982506386 countsByYear W19825063862012 @default.
• W1982506386 countsByYear W19825063862014 @default.
• W1982506386 countsByYear W19825063862015 @default.
• W1982506386 countsByYear W19825063862021 @default.
• W1982506386 crossrefType "journal-article" @default.
• W1982506386 hasAuthorship W1982506386A5058088923 @default.
• W1982506386 hasAuthorship W1982506386A5068711541 @default.
• W1982506386 hasAuthorship W1982506386A5078187729 @default.
• W1982506386 hasConcept C129312508 @default.
• W1982506386 hasConcept C134306372 @default.
• W1982506386 hasConcept C181199279 @default.
• W1982506386 hasConcept C185592680 @default.
• W1982506386 hasConcept C197336794 @default.
• W1982506386 hasConcept C21951064 @default.
• W1982506386 hasConcept C2776043002 @default.
• W1982506386 hasConcept C2776109719 @default.
• W1982506386 hasConcept C33923547 @default.
• W1982506386 hasConcept C552990157 @default.
• W1982506386 hasConcept C55493867 @default.
• W1982506386 hasConcept C71240020 @default.
• W1982506386 hasConceptScore W1982506386C129312508 @default.
• W1982506386 hasConceptScore W1982506386C134306372 @default.
• W1982506386 hasConceptScore W1982506386C181199279 @default.
• W1982506386 hasConceptScore W1982506386C185592680 @default.
• W1982506386 hasConceptScore W1982506386C197336794 @default.
• W1982506386 hasConceptScore W1982506386C21951064 @default.
• W1982506386 hasConceptScore W1982506386C2776043002 @default.
• W1982506386 hasConceptScore W1982506386C2776109719 @default.
• W1982506386 hasConceptScore W1982506386C33923547 @default.
• W1982506386 hasConceptScore W1982506386C552990157 @default.
• W1982506386 hasConceptScore W1982506386C55493867 @default.
• W1982506386 hasConceptScore W1982506386C71240020 @default.
• W1982506386 hasIssue "37" @default.
• W1982506386 hasLocation W19825063861 @default.
• W1982506386 hasOpenAccess W1982506386 @default.
• W1982506386 hasPrimaryLocation W19825063861 @default.
• W1982506386 hasRelatedWork W1970602452 @default.
• W1982506386 hasRelatedWork W2010679856 @default.
• W1982506386 hasRelatedWork W2010766347 @default.
• W1982506386 hasRelatedWork W2032489032 @default.
• W1982506386 hasRelatedWork W2048362237 @default.
• W1982506386 hasRelatedWork W2055833539 @default.
• W1982506386 hasRelatedWork W2134721065 @default.
• W1982506386 hasRelatedWork W2135021230 @default.
• W1982506386 hasRelatedWork W2152511840 @default.
• W1982506386 hasRelatedWork W2952429266 @default.
• W1982506386 hasVolume "52" @default.
• W1982506386 isParatext "false" @default.
• W1982506386 isRetracted "false" @default.
• W1982506386 magId "1982506386" @default.
• W1982506386 workType "article" @default.