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- W1982508575 abstract "Summary The influence of the cytoskeleton on the invasion of Aeromonas hydrophila strain AhJ‐1, isolated from diseased fish, in the monolayer cell of epithelioma papillosum cells of carp (EPC) was evaluated by the recovery of gentamicin‐resistant bacteria from Triton X‐100 cell lysates. The depolymerization of microfilaments (MF) by cytochalasin B and D inhibited the uptake of A. hydrophila in a dose‐dependent manner and that of microtubules (MT) by colchicines and nocodazole did not affect the invasion of A. hydrophila in EPC cells significantly. The invasion frequency decreased approximately 62% with the addition of 0.1 μ g/ml cytochalasin D and nearly 86% by the addition of 5.0 μ g/ml. Invasion decreased approximately 49% and 83% by addition of cytochalasin B in a concentration of 2.5 μ g/ml and 10.0 μ g/ml. Colchicine and nocodazole, inhibitors of MT formation appears to have little effect on the invasion of EPC cells by strain Ah J‐1. Thus MF formation, but not MT formation seems to play an important role in the internalization of A. hydrophila J‐1." @default.
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- W1982508575 date "2005-05-01" @default.
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- W1982508575 title "Role of Microfilaments and Microtubules in the Invasion of EPC Cells by Aeromonas hydrophila" @default.
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- W1982508575 doi "https://doi.org/10.1111/j.1439-0450.2005.00842.x" @default.
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