Matches in SemOpenAlex for { <https://semopenalex.org/work/W1982584829> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W1982584829 abstract "Caprine alpha-2-macroglobulin (alpha2M) is a broad-spectrum, homotetrameric proteinase inhibitor that can maximally bind a single molecule of proteinase. Inhibition of proteinases by caprine alpha2M results from a series of conformational changes that are initiated by the proteinase and results in physical sequestration of the proteinase within the closed cage-like structure of conformationally altered alpha2M. In a previous study, uric acid-generated superoxide anion was identified as one of the physiologically relevant inactivators of alpha2M S.A. Khan, F.H. Khan [Free. Radic. Res. 34 (2001) 113]. We now demonstrate that hypochlorous acid (HOCl) and, to lesser extent, hydrogen peroxide (H2O2) destroy the antiproteolytic potential of caprine alpha2M. At physiologically attainable concentration, we found that HOCl significantly compromised functional integrity of the inhibitor. High concentrations of H2O2 also partially diminished proteinase inhibitory capacity of alpha2M by a mechanism not involving formation of hydroxyl radicals. For hydrogen peroxide, catalase completely protected alpha2M activity while the ability to protect the inhibitor from HOCl-induced inactivation was limited by availability of albumin. Structure function analysis demonstrated that oxidized caprine inhibitor, unlike its human counterpart, retained its tetrameric configuration as well as its characteristic ability to undergo major conformational change upon trypsinization. It is proposed that inhibition of alpha2M activity may be due to oxidation of essential residues of the inhibitor and/or structural rearrangement of the subunits." @default.
- W1982584829 created "2016-06-24" @default.
- W1982584829 creator A5026066843 @default.
- W1982584829 creator A5054833047 @default.
- W1982584829 date "2004-07-01" @default.
- W1982584829 modified "2023-09-26" @default.
- W1982584829 title "Oxidized caprine alpha-2-macroglobulin: damaged but not completely dysfunctional" @default.
- W1982584829 cites W1504842322 @default.
- W1982584829 cites W1576487122 @default.
- W1982584829 cites W1592948498 @default.
- W1982584829 cites W1599542520 @default.
- W1982584829 cites W1759706356 @default.
- W1982584829 cites W1787722527 @default.
- W1982584829 cites W1917517429 @default.
- W1982584829 cites W1925799544 @default.
- W1982584829 cites W1975962599 @default.
- W1982584829 cites W1982890735 @default.
- W1982584829 cites W2007427985 @default.
- W1982584829 cites W2010094123 @default.
- W1982584829 cites W2019524205 @default.
- W1982584829 cites W2024066561 @default.
- W1982584829 cites W2030683196 @default.
- W1982584829 cites W2040447429 @default.
- W1982584829 cites W2042732987 @default.
- W1982584829 cites W2044329953 @default.
- W1982584829 cites W2046005857 @default.
- W1982584829 cites W2046690044 @default.
- W1982584829 cites W2052562838 @default.
- W1982584829 cites W2066981727 @default.
- W1982584829 cites W2072085765 @default.
- W1982584829 cites W2075098204 @default.
- W1982584829 cites W2081312332 @default.
- W1982584829 cites W2085858063 @default.
- W1982584829 cites W2087593387 @default.
- W1982584829 cites W2089151514 @default.
- W1982584829 cites W2100837269 @default.
- W1982584829 cites W2107919301 @default.
- W1982584829 cites W2118127884 @default.
- W1982584829 cites W2131190922 @default.
- W1982584829 cites W2142540364 @default.
- W1982584829 cites W2148555918 @default.
- W1982584829 cites W2154658221 @default.
- W1982584829 cites W2171615170 @default.
- W1982584829 cites W2177092266 @default.
- W1982584829 cites W2196966592 @default.
- W1982584829 cites W2260835883 @default.
- W1982584829 cites W2312389273 @default.
- W1982584829 cites W2314530439 @default.
- W1982584829 cites W2072796605 @default.
- W1982584829 cites W2076079559 @default.
- W1982584829 doi "https://doi.org/10.1016/j.bbagen.2004.06.008" @default.
- W1982584829 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15374618" @default.
- W1982584829 hasPublicationYear "2004" @default.
- W1982584829 type Work @default.
- W1982584829 sameAs 1982584829 @default.
- W1982584829 citedByCount "3" @default.
- W1982584829 countsByYear W19825848292013 @default.
- W1982584829 countsByYear W19825848292018 @default.
- W1982584829 crossrefType "journal-article" @default.
- W1982584829 hasAuthorship W1982584829A5026066843 @default.
- W1982584829 hasAuthorship W1982584829A5054833047 @default.
- W1982584829 hasConcept C181199279 @default.
- W1982584829 hasConcept C185592680 @default.
- W1982584829 hasConcept C2778979269 @default.
- W1982584829 hasConcept C2780908991 @default.
- W1982584829 hasConcept C533411734 @default.
- W1982584829 hasConcept C55493867 @default.
- W1982584829 hasConcept C71240020 @default.
- W1982584829 hasConceptScore W1982584829C181199279 @default.
- W1982584829 hasConceptScore W1982584829C185592680 @default.
- W1982584829 hasConceptScore W1982584829C2778979269 @default.
- W1982584829 hasConceptScore W1982584829C2780908991 @default.
- W1982584829 hasConceptScore W1982584829C533411734 @default.
- W1982584829 hasConceptScore W1982584829C55493867 @default.
- W1982584829 hasConceptScore W1982584829C71240020 @default.
- W1982584829 hasLocation W19825848291 @default.
- W1982584829 hasLocation W19825848292 @default.
- W1982584829 hasOpenAccess W1982584829 @default.
- W1982584829 hasPrimaryLocation W19825848291 @default.
- W1982584829 hasRelatedWork W175681964 @default.
- W1982584829 hasRelatedWork W1966368483 @default.
- W1982584829 hasRelatedWork W1998528036 @default.
- W1982584829 hasRelatedWork W2016696944 @default.
- W1982584829 hasRelatedWork W2116012372 @default.
- W1982584829 hasRelatedWork W2159780227 @default.
- W1982584829 hasRelatedWork W2378240399 @default.
- W1982584829 hasRelatedWork W2474045390 @default.
- W1982584829 hasRelatedWork W4210583805 @default.
- W1982584829 hasRelatedWork W964043021 @default.
- W1982584829 isParatext "false" @default.
- W1982584829 isRetracted "false" @default.
- W1982584829 magId "1982584829" @default.
- W1982584829 workType "article" @default.