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- W1982607602 abstract "Jack pine seedlings rapidly converted radioactive thymine-6- 3 H to dihydrothymine, β-ureidoisobutyric acid, and β-aminoisobutyric acid. Autoradiographs of hypocotyl cells showed radioactivity largely in the cytoplasm. A small percentage of radioactivity from thymine-2- 14 C was salvaged via uracil for the synthesis of RNA. DNA was not labelled significantly. The occurrence of a mechanism to utilize thymidine-methyl- 3 H for DNA synthesis was detected at low levels in germinating seedlings but was absent in seeds up to 16 h imbibition. Among pyrimidines tested thymidine was the most specific for the labelling of DNA and radioactivity was detected almost entirely over nuclei.The occurrence of an active pathway for the de novo synthesis of the pyrimidine portions of nucleic acids was demonstrated by the use of orotic acid-4- 14 C. Native DNA and all major types of RNA were labelled and radioactivity resided in pyrimidines. Synthesis of DNA involved the conversion of pyrimidine ribonucleotides to deoxyribonucleotides." @default.
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- W1982607602 date "1975-04-01" @default.
- W1982607602 modified "2023-09-25" @default.
- W1982607602 title "Pyrimidine metabolism in seeds and seedlings of jack pine (Pinus banksiana)" @default.
- W1982607602 doi "https://doi.org/10.1139/b75-083" @default.
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