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- W1982667734 abstract "Abstract The allelic polymorphism and inheritance of Md ACS 1 and Md ACO 1 genes were analysed in 28 apple cultivars, which were derived from reciprocal crosses of the following parental pairs: ‘Golden Delicious’ × ‘James Grieve’, ‘Golden Delicious’ × ‘Jonathan’, ‘Cox's Orange Pippin’ × ‘Golden Delicious’, ‘Cox's Orange Pippin’ × ‘Jonathan’. Polymorphisms were detected by PCR and the use of two restriction enzymes ( Bam H1 and Rsa I). In addition, new primers were designed for the further discrimination of Md ACO 1 alleles. Two alleles of Md ACS 1 gene ( Md ACS 1‐1 and Md ACS 1‐2 ) and three alleles of Md ACO 1 gene ( a , b and c ) were detected. Cloning and sequencing of Md ACO 1 alleles confirmed a high conservation and some differences within the coding regions and helped to reconcile between different numbering systems. Observed segregations confirmed that alleles a , b and c belonged to the Md ACO 1 gene. It is apparent that polymorphisms within the Md ACS 1 and Md ACO 1 genes could aid cultivar genotyping and identification and, furthermore, that the Md ACS 1‐2/2 genotype is correlated with a long apple storage life." @default.
- W1982667734 created "2016-06-24" @default.
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- W1982667734 date "2013-12-05" @default.
- W1982667734 modified "2023-10-03" @default.
- W1982667734 title "Allelic polymorphism and inheritance of<i>MdACS1</i>and<i>MdACO1</i>genes in apple (<i>Malus </i>× <i>domestica</i>Borkh.)" @default.
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- W1982667734 doi "https://doi.org/10.1111/pbr.12124" @default.
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