FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1982697547> ?p ?o ?g. }

• W1982697547 endingPage "2780" @default.
• W1982697547 startingPage "2774" @default.
• W1982697547 abstract "Lipoxygenases (LOX) form a heterogeneous family of lipid peroxidizing enzymes, which catalyze specific dioxygenation of polyunsaturated fatty acids. According to their positional specificity of linoleic acid oxygenation plant LOX have been classified into linoleate 9- and linoleate 13-LOX and recent reports identified a critical valine at the active site of 9-LOX. In contrast, more bulky phenylalanine or histidine residues were found at this position in 13-LOX. We have recently cloned a LOX-isoform from Momordica charantia and multiple amino acid alignments indicated the existence of a glutamine (Gln599) at the position were 13-LOX usually carry histidine or phenylalanine residues. Analyzing the pH-dependence of the positional specificity of linoleic acid oxygenation we observed that at pH-values higher than 7.5 this enzyme constitutes a linoleate 13-LOX whereas at lower pH, 9-H(P)ODE was the major reaction product. Site-directed mutagenesis of glutamine 599 to histidine (Gln599His) converted the enzyme to a pure 13-LOX. These data confirm previous observation suggesting that reaction specificity of certain LOX-isoforms is not an absolute enzyme property but may be impacted by reaction conditions such as pH of the reaction mixture. We extended this concept by identifying glutamine 599 as sequence determinant for such pH-dependence of the reaction specificity. Although the biological relevance for this alteration switch remains to be investigated it is of particular interest that it occurs at near physiological conditions in the pH-range between 7 and 8." @default.
• W1982697547 created "2016-06-24" @default.
• W1982697547 creator A5012844266 @default.
• W1982697547 creator A5014546503 @default.
• W1982697547 creator A5018960820 @default.
• W1982697547 creator A5052995713 @default.
• W1982697547 creator A5053379778 @default.
• W1982697547 creator A5061259217 @default.
• W1982697547 creator A5064301762 @default.
• W1982697547 creator A5082493714 @default.
• W1982697547 creator A5089220465 @default.
• W1982697547 date "2008-11-01" @default.
• W1982697547 modified "2023-10-16" @default.
• W1982697547 title "Identification of an amino acid determinant of pH regiospecificity in a seed lipoxygenase from Momordica charantia" @default.
• W1982697547 cites W1520913485 @default.
• W1982697547 cites W1531198291 @default.
• W1982697547 cites W1978250288 @default.
• W1982697547 cites W1978704058 @default.
• W1982697547 cites W1979892619 @default.
• W1982697547 cites W1984510490 @default.
• W1982697547 cites W1995385088 @default.
• W1982697547 cites W1998036446 @default.
• W1982697547 cites W2000216296 @default.
• W1982697547 cites W2016840330 @default.
• W1982697547 cites W2043320986 @default.
• W1982697547 cites W2052624151 @default.
• W1982697547 cites W2074203890 @default.
• W1982697547 cites W2096719701 @default.
• W1982697547 cites W2103146644 @default.
• W1982697547 cites W2113000402 @default.
• W1982697547 cites W2119280206 @default.
• W1982697547 cites W2127492873 @default.
• W1982697547 cites W2143251805 @default.
• W1982697547 cites W2147705263 @default.
• W1982697547 cites W2152311269 @default.
• W1982697547 cites W2163782474 @default.
• W1982697547 cites W2169076235 @default.
• W1982697547 cites W3191402597 @default.
• W1982697547 doi "https://doi.org/10.1016/j.phytochem.2008.09.006" @default.
• W1982697547 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18945457" @default.
• W1982697547 hasPublicationYear "2008" @default.
• W1982697547 type Work @default.
• W1982697547 sameAs 1982697547 @default.
• W1982697547 citedByCount "18" @default.
• W1982697547 countsByYear W19826975472012 @default.
• W1982697547 countsByYear W19826975472015 @default.
• W1982697547 countsByYear W19826975472018 @default.
• W1982697547 countsByYear W19826975472019 @default.
• W1982697547 countsByYear W19826975472021 @default.
• W1982697547 countsByYear W19826975472023 @default.
• W1982697547 crossrefType "journal-article" @default.
• W1982697547 hasAuthorship W1982697547A5012844266 @default.
• W1982697547 hasAuthorship W1982697547A5014546503 @default.
• W1982697547 hasAuthorship W1982697547A5018960820 @default.
• W1982697547 hasAuthorship W1982697547A5052995713 @default.
• W1982697547 hasAuthorship W1982697547A5053379778 @default.
• W1982697547 hasAuthorship W1982697547A5061259217 @default.
• W1982697547 hasAuthorship W1982697547A5064301762 @default.
• W1982697547 hasAuthorship W1982697547A5082493714 @default.
• W1982697547 hasAuthorship W1982697547A5089220465 @default.
• W1982697547 hasConcept C181199279 @default.
• W1982697547 hasConcept C185592680 @default.
• W1982697547 hasConcept C2777431362 @default.
• W1982697547 hasConcept C2777573094 @default.
• W1982697547 hasConcept C2778460671 @default.
• W1982697547 hasConcept C2778686829 @default.
• W1982697547 hasConcept C2779349466 @default.
• W1982697547 hasConcept C2780809830 @default.
• W1982697547 hasConcept C2780857362 @default.
• W1982697547 hasConcept C41183919 @default.
• W1982697547 hasConcept C515207424 @default.
• W1982697547 hasConcept C543025807 @default.
• W1982697547 hasConcept C55493867 @default.
• W1982697547 hasConcept C556039675 @default.
• W1982697547 hasConcept C71240020 @default.
• W1982697547 hasConcept C71924100 @default.
• W1982697547 hasConceptScore W1982697547C181199279 @default.
• W1982697547 hasConceptScore W1982697547C185592680 @default.
• W1982697547 hasConceptScore W1982697547C2777431362 @default.
• W1982697547 hasConceptScore W1982697547C2777573094 @default.
• W1982697547 hasConceptScore W1982697547C2778460671 @default.
• W1982697547 hasConceptScore W1982697547C2778686829 @default.
• W1982697547 hasConceptScore W1982697547C2779349466 @default.
• W1982697547 hasConceptScore W1982697547C2780809830 @default.
• W1982697547 hasConceptScore W1982697547C2780857362 @default.
• W1982697547 hasConceptScore W1982697547C41183919 @default.
• W1982697547 hasConceptScore W1982697547C515207424 @default.
• W1982697547 hasConceptScore W1982697547C543025807 @default.
• W1982697547 hasConceptScore W1982697547C55493867 @default.
• W1982697547 hasConceptScore W1982697547C556039675 @default.
• W1982697547 hasConceptScore W1982697547C71240020 @default.
• W1982697547 hasConceptScore W1982697547C71924100 @default.
• W1982697547 hasIssue "16" @default.
• W1982697547 hasLocation W19826975471 @default.
• W1982697547 hasLocation W19826975472 @default.
• W1982697547 hasOpenAccess W1982697547 @default.
• W1982697547 hasPrimaryLocation W19826975471 @default.
• W1982697547 hasRelatedWork W1999146778 @default.

• next