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- W1982730514 abstract "The venom of the scorpion, Tityus serrulatus , was fractionated to investigate the chemical and pharmacological properties of its α‐toxin content. Three α‐toxins (Ts III, Ts IV and Ts V) were purified by conventional chromatography (gel filtration and ion‐exchange chromatography), followed by immunoaffinity chromatography. Competition experiments using reference α‐ and β‐toxins suggested that these α‐toxins were contaminated with around 0.01% of β‐toxin. The sequence of the first 30 amino acids of Ts IV was established. Using an oligonucleotide probe, a cDNA encoding its precursor was cloned from a venom gland cDNA library. The primary structure deduced from the cDNA nucleotide sequence provides possible explanations for the polymorphism of these three molecules." @default.
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- W1982730514 date "1994-04-04" @default.
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- W1982730514 title "Biochemical, pharmacological and genomic characterisation of Ts IV, an α-toxin from the venom of the South American scorpion<i>Tityus serrulatus</i>" @default.
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- W1982730514 doi "https://doi.org/10.1016/0014-5793(94)80496-6" @default.
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