FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1982863905> ?p ?o ?g. }

• W1982863905 endingPage "1107" @default.
• W1982863905 startingPage "1089" @default.
• W1982863905 abstract "The site-specific DNA recombinase, gammadelta resolvase, from Escherichia coli catalyzes recombination of res site-containing plasmid DNA to two catenated circular DNA products. The catalytic domain (residues 1-105), lacking a C-terminal dimerization interface, has been constructed and the NMR solution structure of the monomer determined. The RMSD of the NMR conformers for residues 2-92 excluding residues 37-45 and 64-73 is 0.41 A for backbone atoms and 0.88 A for all heavy atoms. The NMR solution structure of the monomeric catalytic domain (residues 1-105) was found to be formed by a four-stranded parallel beta-sheet surrounded by three helices. The catalytic domain (residues 1-105), deficient in the C-terminal dimerization domain, was monomeric at high salt concentration, but displayed unexpected dimerization at lower ionic strength. The unique solution dimerization interface at low ionic strength was mapped by NMR. With respect to previous crystal structures of the dimeric catalytic domain (residues 1-140), differences in the average conformation of active-site residues were found at loop 1 containing the catalytic S10 nucleophile, the beta1 strand containing R8, and at loop 3 containing D67, R68 and R71, which are required for catalysis. The active-site loops display high-frequency and conformational backbone dynamics and are less well defined than the secondary structures. In the solution structure, the D67 side-chain is proximal to the S10 side-chain making the D67 carboxylate group a candidate for activation of S10 through general base catalysis. Four conserved Arg residues can function in the activation of the phosphodiester for nucleophilic attack by the S10 hydroxyl group. A mechanism for covalent catalysis by this class of recombinases is proposed that may be related to dimer interface dissociation." @default.
• W1982863905 created "2016-06-24" @default.
• W1982863905 creator A5025815678 @default.
• W1982863905 creator A5027190807 @default.
• W1982863905 creator A5036926999 @default.
• W1982863905 creator A5075089490 @default.
• W1982863905 date "2001-07-01" @default.
• W1982863905 modified "2023-09-29" @default.
• W1982863905 title "Solution structure of the catalytic domain of γδ resolvase. Implications for the mechanism of catalysis 1 1Edited by P. E. Wright" @default.
• W1982863905 cites W1032034317 @default.
• W1982863905 cites W11005829 @default.
• W1982863905 cites W139456597 @default.
• W1982863905 cites W1488316916 @default.
• W1982863905 cites W1519899019 @default.
• W1982863905 cites W1584594367 @default.
• W1982863905 cites W1587060900 @default.
• W1982863905 cites W1590546489 @default.
• W1982863905 cites W168488792 @default.
• W1982863905 cites W1752095998 @default.
• W1982863905 cites W1932828446 @default.
• W1982863905 cites W1970494673 @default.
• W1982863905 cites W1987587101 @default.
• W1982863905 cites W1989562028 @default.
• W1982863905 cites W1993877983 @default.
• W1982863905 cites W2000948557 @default.
• W1982863905 cites W2002195659 @default.
• W1982863905 cites W2008045976 @default.
• W1982863905 cites W2017778575 @default.
• W1982863905 cites W2019451098 @default.
• W1982863905 cites W2022058405 @default.
• W1982863905 cites W2030159756 @default.
• W1982863905 cites W2040650484 @default.
• W1982863905 cites W2040953733 @default.
• W1982863905 cites W2048200733 @default.
• W1982863905 cites W2052933818 @default.
• W1982863905 cites W2058589767 @default.
• W1982863905 cites W2061144516 @default.
• W1982863905 cites W2062715753 @default.
• W1982863905 cites W2064873329 @default.
• W1982863905 cites W2065859262 @default.
• W1982863905 cites W2073206041 @default.
• W1982863905 cites W2078553109 @default.
• W1982863905 cites W2078801039 @default.
• W1982863905 cites W2080437983 @default.
• W1982863905 cites W2120412445 @default.
• W1982863905 cites W2124408385 @default.
• W1982863905 cites W2126489217 @default.
• W1982863905 cites W2129879061 @default.
• W1982863905 cites W2137434323 @default.
• W1982863905 cites W2139798079 @default.
• W1982863905 cites W2141399204 @default.
• W1982863905 cites W2152942816 @default.
• W1982863905 cites W2158143441 @default.
• W1982863905 cites W2159318560 @default.
• W1982863905 cites W2166923082 @default.
• W1982863905 cites W2169303193 @default.
• W1982863905 cites W2171184423 @default.
• W1982863905 cites W2416859981 @default.
• W1982863905 cites W29362848 @default.
• W1982863905 cites W383695483 @default.
• W1982863905 cites W4230765459 @default.
• W1982863905 cites W4234411972 @default.
• W1982863905 cites W568144879 @default.
• W1982863905 doi "https://doi.org/10.1006/jmbi.2001.4821" @default.
• W1982863905 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11501998" @default.
• W1982863905 hasPublicationYear "2001" @default.
• W1982863905 type Work @default.
• W1982863905 sameAs 1982863905 @default.
• W1982863905 citedByCount "22" @default.
• W1982863905 countsByYear W19828639052012 @default.
• W1982863905 countsByYear W19828639052014 @default.
• W1982863905 countsByYear W19828639052015 @default.
• W1982863905 countsByYear W19828639052016 @default.
• W1982863905 countsByYear W19828639052017 @default.
• W1982863905 countsByYear W19828639052018 @default.
• W1982863905 crossrefType "journal-article" @default.
• W1982863905 hasAuthorship W1982863905A5025815678 @default.
• W1982863905 hasAuthorship W1982863905A5027190807 @default.
• W1982863905 hasAuthorship W1982863905A5036926999 @default.
• W1982863905 hasAuthorship W1982863905A5075089490 @default.
• W1982863905 hasConcept C104317684 @default.
• W1982863905 hasConcept C105793898 @default.
• W1982863905 hasConcept C143065580 @default.
• W1982863905 hasConcept C161790260 @default.
• W1982863905 hasConcept C166940927 @default.
• W1982863905 hasConcept C178790620 @default.
• W1982863905 hasConcept C185592680 @default.
• W1982863905 hasConcept C47701112 @default.
• W1982863905 hasConcept C4918238 @default.
• W1982863905 hasConcept C521977710 @default.
• W1982863905 hasConcept C55493867 @default.
• W1982863905 hasConcept C71240020 @default.
• W1982863905 hasConcept C8010536 @default.
• W1982863905 hasConceptScore W1982863905C104317684 @default.
• W1982863905 hasConceptScore W1982863905C105793898 @default.
• W1982863905 hasConceptScore W1982863905C143065580 @default.
• W1982863905 hasConceptScore W1982863905C161790260 @default.
• W1982863905 hasConceptScore W1982863905C166940927 @default.

• next