FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1982877488> ?p ?o ?g. }

• W1982877488 endingPage "e231" @default.
• W1982877488 startingPage "e221" @default.
• W1982877488 abstract "Protein kinase G (PKG), a recognized downstream mediator of nitric oxide, is a key regulator of cardiovascular physiology and pathology. High-level stimulation of cyclic guanosine monophosphate/PKG signaling using high concentrations of nitric oxide donors, mimicking pathological conditions, induces apoptosis in vascular smooth muscle cells. In contrast, we have found that PKG at basal and moderately elevated activity prevents both spontaneous and toxin-induced apoptosis in many other cells. We hypothesized that PKG's apoptosis-regulatory role in vascular smooth muscle cells depends on PKG activation levels [low/basal-level activation prevents apoptosis, whereas high-level activation (hyperactivation) causes apoptosis]. Furthermore, we hypothesized that, although PKG hyperactivation inhibits vascular smooth muscle cell proliferation (potentially causing anti-atherogenic effects), basal PKG activity may promote vascular smooth muscle cell proliferation/atherogenesis. Involvement of PKG in apoptosis and proliferation was determined in unpassaged vascular smooth muscle cells from mouse aorta. Western blot analysis was used to determine PKG expression, and activators/inhibitors of PKG activity were used to determine involvement in apoptosis (Hoechst staining and DNA-fragmentation ELISAs) and proliferation (cell count, MTT assay, and BrdU incorporation). Both PKG-Iα and PKG-Iβ isoforms were expressed. Lower-level stimulation of PKG using the nitric oxide donor S-nitroso-acetylpenacillamine (10, 50 μM) significantly (P<.05) lowered spontaneous apoptosis, whereas S-nitroso-acetylpenacillamine at higher concentrations (500, 1000 μM) elevated apoptosis. Twenty-four-hour pretreatment with atrial natriuretic peptide, a PKG activator, completely prevented high-concentration, nitric oxide-induced apoptosis. Inhibition of basal PKG activity using highly selective PKG inhibitors, DT-2 and DT-3, significantly (P<.001) increased apoptosis and inhibited DNA synthesis/proliferation. The data suggest that basal/moderately elevated PKG activity protects against high/pathological-level nitric oxide-induced apoptosis and promotes DNA synthesis/proliferation in vascular smooth muscle cells, potentially important for atherogenesis." @default.
• W1982877488 created "2016-06-24" @default.
• W1982877488 creator A5057388371 @default.
• W1982877488 creator A5076993163 @default.
• W1982877488 date "2010-11-01" @default.
• W1982877488 modified "2023-10-10" @default.
• W1982877488 title "Protein kinase G activity prevents pathological-level nitric oxide-induced apoptosis and promotes DNA synthesis/cell proliferation in vascular smooth muscle cells" @default.
• W1982877488 cites W1012224914 @default.
• W1982877488 cites W1529543999 @default.
• W1982877488 cites W1821918060 @default.
• W1982877488 cites W1970366992 @default.
• W1982877488 cites W1974261894 @default.
• W1982877488 cites W1977887865 @default.
• W1982877488 cites W1980458823 @default.
• W1982877488 cites W1989526884 @default.
• W1982877488 cites W1990197131 @default.
• W1982877488 cites W1990207368 @default.
• W1982877488 cites W1992976892 @default.
• W1982877488 cites W1994807255 @default.
• W1982877488 cites W1994958067 @default.
• W1982877488 cites W1996406153 @default.
• W1982877488 cites W2002083717 @default.
• W1982877488 cites W2005398476 @default.
• W1982877488 cites W2010336604 @default.
• W1982877488 cites W2026247591 @default.
• W1982877488 cites W2033529194 @default.
• W1982877488 cites W2040663949 @default.
• W1982877488 cites W2042288688 @default.
• W1982877488 cites W2042736881 @default.
• W1982877488 cites W2054071295 @default.
• W1982877488 cites W2062770053 @default.
• W1982877488 cites W2067486773 @default.
• W1982877488 cites W2074224605 @default.
• W1982877488 cites W2076618504 @default.
• W1982877488 cites W2077082072 @default.
• W1982877488 cites W2079129494 @default.
• W1982877488 cites W2080573406 @default.
• W1982877488 cites W2082023944 @default.
• W1982877488 cites W2083043369 @default.
• W1982877488 cites W2083647583 @default.
• W1982877488 cites W2084349854 @default.
• W1982877488 cites W2092585489 @default.
• W1982877488 cites W2095186122 @default.
• W1982877488 cites W2100657656 @default.
• W1982877488 cites W2109679295 @default.
• W1982877488 cites W2118440238 @default.
• W1982877488 cites W2125457799 @default.
• W1982877488 cites W2130400938 @default.
• W1982877488 cites W2130506421 @default.
• W1982877488 cites W2139707112 @default.
• W1982877488 cites W2144650531 @default.
• W1982877488 cites W2150832632 @default.
• W1982877488 cites W84704252 @default.
• W1982877488 doi "https://doi.org/10.1016/j.carpath.2009.11.001" @default.
• W1982877488 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20060325" @default.
• W1982877488 hasPublicationYear "2010" @default.
• W1982877488 type Work @default.
• W1982877488 sameAs 1982877488 @default.
• W1982877488 citedByCount "18" @default.
• W1982877488 countsByYear W19828774882012 @default.
• W1982877488 countsByYear W19828774882013 @default.
• W1982877488 countsByYear W19828774882015 @default.
• W1982877488 countsByYear W19828774882016 @default.
• W1982877488 countsByYear W19828774882017 @default.
• W1982877488 countsByYear W19828774882018 @default.
• W1982877488 countsByYear W19828774882019 @default.
• W1982877488 countsByYear W19828774882020 @default.
• W1982877488 countsByYear W19828774882022 @default.
• W1982877488 countsByYear W19828774882023 @default.
• W1982877488 crossrefType "journal-article" @default.
• W1982877488 hasAuthorship W1982877488A5057388371 @default.
• W1982877488 hasAuthorship W1982877488A5076993163 @default.
• W1982877488 hasConcept C126322002 @default.
• W1982877488 hasConcept C134018914 @default.
• W1982877488 hasConcept C173803235 @default.
• W1982877488 hasConcept C184235292 @default.
• W1982877488 hasConcept C185592680 @default.
• W1982877488 hasConcept C190283241 @default.
• W1982877488 hasConcept C199217515 @default.
• W1982877488 hasConcept C2778827303 @default.
• W1982877488 hasConcept C2779395532 @default.
• W1982877488 hasConcept C2780994212 @default.
• W1982877488 hasConcept C2992686903 @default.
• W1982877488 hasConcept C519581460 @default.
• W1982877488 hasConcept C55493867 @default.
• W1982877488 hasConcept C71924100 @default.
• W1982877488 hasConcept C82495950 @default.
• W1982877488 hasConcept C86803240 @default.
• W1982877488 hasConcept C95444343 @default.
• W1982877488 hasConcept C97029542 @default.
• W1982877488 hasConceptScore W1982877488C126322002 @default.
• W1982877488 hasConceptScore W1982877488C134018914 @default.
• W1982877488 hasConceptScore W1982877488C173803235 @default.
• W1982877488 hasConceptScore W1982877488C184235292 @default.
• W1982877488 hasConceptScore W1982877488C185592680 @default.
• W1982877488 hasConceptScore W1982877488C190283241 @default.
• W1982877488 hasConceptScore W1982877488C199217515 @default.
• W1982877488 hasConceptScore W1982877488C2778827303 @default.

• next