Matches in SemOpenAlex for { <https://semopenalex.org/work/W1982887137> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W1982887137 endingPage "235" @default.
- W1982887137 startingPage "233" @default.
- W1982887137 abstract "Although several protocols were developed to extract DNA for soil-transmitted helminthiasis diagnostic, amplifying these extracts remains challenging due to DNA polymerase inhibitors. This study aimed to assess a DNA extraction method for efficient detection of soil-transmitted helminth species by determining stool mass and the type of DNA polymerase that can be used for this extraction method. For this study, 141 stool samples harbouring soil-transmitted eggs and 50 samples without egg were obtained from school-aged children of Makenene in the Centre region of Cameroon. DNA was extracted from 10, 20, 40 and 80 mg of stool using commercial kit and/or cetyltrimethylammonium bromide (CTAB)-based method. The amount of stool for molecular diagnostic of soil-transmitted helminthiasis was determined by amplifying Ascaris lumbricoides DNA. The performances of three DNA polymerases and CTAB-based method were assessed by amplifying DNA of different soil-transmitted helminth species. For this study, 94 stools with A. lumbricoides eggs, 39 with Trichuris trichuria and 15 with hookworm were analyzed. DNA of A. lumbricoides, T. trichuria, Necator americanus and Ancylostoma duodenale were detected in 97.9% of extracts from stools harbouring soil-transmitted helminth eggs. Soil-transmitted helminth DNAs were significantly (X2 = 17.66; df = 3; p 〈00001) more amplified in extracts from 10 and 20 mg than those from 40 and 80 mg. The amplification rate with “Q5 high fidelity DNA polymerase” was significantly (X2 = 30.54; df = 2; p < 0.00001) higher than that of other DNA polymerases. Multiplex-PCR confirmed co-infections of A. lumbricoides with either T. trichuria or N. americanus. The extraction cost for the CTAB-based method was $1.45. This method appearedis reliable and 3 times cost effective than commercial kit. Its combination with the “Q5 high fidelity DNA polymerase” may improve soil-transmitted helminthiasis diagnostic." @default.
- W1982887137 created "2016-06-24" @default.
- W1982887137 creator A5007618526 @default.
- W1982887137 creator A5008185837 @default.
- W1982887137 creator A5021779667 @default.
- W1982887137 creator A5035791741 @default.
- W1982887137 creator A5065038228 @default.
- W1982887137 date "1997-10-01" @default.
- W1982887137 modified "2023-10-16" @default.
- W1982887137 title "A fast CTAB method of human DNA isolation for polymerase chain reaction applications" @default.
- W1982887137 cites W1983368570 @default.
- W1982887137 cites W84802023 @default.
- W1982887137 doi "https://doi.org/10.1016/s0307-4412(97)00122-2" @default.
- W1982887137 hasPublicationYear "1997" @default.
- W1982887137 type Work @default.
- W1982887137 sameAs 1982887137 @default.
- W1982887137 citedByCount "16" @default.
- W1982887137 countsByYear W19828871372017 @default.
- W1982887137 countsByYear W19828871372019 @default.
- W1982887137 countsByYear W19828871372020 @default.
- W1982887137 countsByYear W19828871372021 @default.
- W1982887137 countsByYear W19828871372023 @default.
- W1982887137 crossrefType "journal-article" @default.
- W1982887137 hasAuthorship W1982887137A5007618526 @default.
- W1982887137 hasAuthorship W1982887137A5008185837 @default.
- W1982887137 hasAuthorship W1982887137A5021779667 @default.
- W1982887137 hasAuthorship W1982887137A5035791741 @default.
- W1982887137 hasAuthorship W1982887137A5065038228 @default.
- W1982887137 hasConcept C104317684 @default.
- W1982887137 hasConcept C153911025 @default.
- W1982887137 hasConcept C165901193 @default.
- W1982887137 hasConcept C2777047024 @default.
- W1982887137 hasConcept C2777364306 @default.
- W1982887137 hasConcept C2777683361 @default.
- W1982887137 hasConcept C2779459945 @default.
- W1982887137 hasConcept C2779786594 @default.
- W1982887137 hasConcept C42972112 @default.
- W1982887137 hasConcept C49105822 @default.
- W1982887137 hasConcept C54355233 @default.
- W1982887137 hasConcept C552990157 @default.
- W1982887137 hasConcept C71924100 @default.
- W1982887137 hasConcept C78063203 @default.
- W1982887137 hasConcept C86803240 @default.
- W1982887137 hasConcept C89423630 @default.
- W1982887137 hasConcept C90583042 @default.
- W1982887137 hasConcept C90856448 @default.
- W1982887137 hasConceptScore W1982887137C104317684 @default.
- W1982887137 hasConceptScore W1982887137C153911025 @default.
- W1982887137 hasConceptScore W1982887137C165901193 @default.
- W1982887137 hasConceptScore W1982887137C2777047024 @default.
- W1982887137 hasConceptScore W1982887137C2777364306 @default.
- W1982887137 hasConceptScore W1982887137C2777683361 @default.
- W1982887137 hasConceptScore W1982887137C2779459945 @default.
- W1982887137 hasConceptScore W1982887137C2779786594 @default.
- W1982887137 hasConceptScore W1982887137C42972112 @default.
- W1982887137 hasConceptScore W1982887137C49105822 @default.
- W1982887137 hasConceptScore W1982887137C54355233 @default.
- W1982887137 hasConceptScore W1982887137C552990157 @default.
- W1982887137 hasConceptScore W1982887137C71924100 @default.
- W1982887137 hasConceptScore W1982887137C78063203 @default.
- W1982887137 hasConceptScore W1982887137C86803240 @default.
- W1982887137 hasConceptScore W1982887137C89423630 @default.
- W1982887137 hasConceptScore W1982887137C90583042 @default.
- W1982887137 hasConceptScore W1982887137C90856448 @default.
- W1982887137 hasIssue "4" @default.
- W1982887137 hasLocation W19828871371 @default.
- W1982887137 hasOpenAccess W1982887137 @default.
- W1982887137 hasPrimaryLocation W19828871371 @default.
- W1982887137 hasRelatedWork W153625009 @default.
- W1982887137 hasRelatedWork W1984488964 @default.
- W1982887137 hasRelatedWork W2253183016 @default.
- W1982887137 hasRelatedWork W2301480749 @default.
- W1982887137 hasRelatedWork W2340423736 @default.
- W1982887137 hasRelatedWork W2343012098 @default.
- W1982887137 hasRelatedWork W2361053895 @default.
- W1982887137 hasRelatedWork W2416737627 @default.
- W1982887137 hasRelatedWork W3213840309 @default.
- W1982887137 hasRelatedWork W2473567640 @default.
- W1982887137 hasVolume "25" @default.
- W1982887137 isParatext "false" @default.
- W1982887137 isRetracted "false" @default.
- W1982887137 magId "1982887137" @default.
- W1982887137 workType "article" @default.