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- W1982897076 abstract "We present an active oligonucleotide microarray platform for time-resolved Förster-resonance-energy-transfer (TR-FRET) assays. In these assays, immobilized probe is labeled with a donor fluorophore and analyte target is labeled with a fluorescence quencher. Changes in the fluorescence decay lifetime of the donor are measured to determine the extent of hybridization. In this work, we demonstrate that TR-FRET assays have reduced sensitivity to variances in probe surface density compared with standard fluorescence-based microarray assays. Use of an active array substrate, fabricated in a standard complementary metal-oxide-semiconductor (CMOS) process, provides the additional benefits of reduced system complexity and cost. The array consists of 4096 independent single-photon avalanche diode (SPAD) pixel sites and features on-chip time-to-digital conversion. We demonstrate the functionality of our system by measuring a DNA target concentration series using TR-FRET with semiconductor quantum dot donors." @default.
- W1982897076 created "2016-06-24" @default.
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- W1982897076 date "2008-11-01" @default.
- W1982897076 modified "2023-10-18" @default.
- W1982897076 title "Time-resolved Förster-resonance-energy-transfer DNA assay on an active CMOS microarray" @default.
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- W1982897076 doi "https://doi.org/10.1016/j.bios.2008.04.015" @default.
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