FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1982925298> ?p ?o ?g. }

• W1982925298 endingPage "6602" @default.
• W1982925298 startingPage "6595" @default.
• W1982925298 abstract "We have developed a transient transfection system using the Cytomegalovirus (CMV) promoter to express the human estrogen receptor (ER) at very high levels in COS-1 cells and have used it to study the interaction of agonist and antagonist receptor complexes with estrogen response element (ERE) DNA. ER can be expressed to levels of 20–40 pmol/mg or 0.2–0.3% of total soluble protein and all of the soluble receptor is capable of binding hormone. The ER binds estradiol with high affinity (Kd 0.2 nM), and is indistinguishable from native ER in that the receptor is capable of recognizing its cognate DNA response element with high affinity, and of transactivating a transgene in an estradiol-dependent manner. Gel mobility shift assays reveal interesting ligand-dependent differences in the binding of receptor complexes to ERE DNA. Receptors occupied by estradiol or the type I antiestrogen transhydroxytamoxifen bind to DNA response elements when exposed to the ligand in vitro or in vivo . Likewise, receptors exposed to the type II antiestrogen ICI 164,384 in vitro bind to ERE DNA. However, when receptor exposure to IC1164,384 is carried out in vivo , the ER-IC1164,384 complexes do not bind to ERE DNA, or do so only weakly. This effect is not reversed by subsequent incubation with estradiol in vitro , but is rapidly reversible by in vivo estradiol exposure of intact COS-1 cells. This suggests there may be some cellular process involved in the mechanism of antagonism by the pure antiestrogen ICI 164,384, which is not observed in cell-free extracts." @default.
• W1982925298 created "2016-06-24" @default.
• W1982925298 creator A5040793693 @default.
• W1982925298 creator A5080531304 @default.
• W1982925298 date "1991-01-01" @default.
• W1982925298 modified "2023-09-23" @default.
• W1982925298 title "Differential DNA-binding abilites of estrogen receptor occupied with two classes of antiestrogens: studies using human estrogen receptor overexpressed in mammalian cells" @default.
• W1982925298 cites W1482432683 @default.
• W1982925298 cites W1516538526 @default.
• W1982925298 cites W1545204276 @default.
• W1982925298 cites W1562960938 @default.
• W1982925298 cites W1585098875 @default.
• W1982925298 cites W1585603261 @default.
• W1982925298 cites W1591964692 @default.
• W1982925298 cites W1670076014 @default.
• W1982925298 cites W1675708121 @default.
• W1982925298 cites W180778085 @default.
• W1982925298 cites W1964520022 @default.
• W1982925298 cites W1965735992 @default.
• W1982925298 cites W1976495127 @default.
• W1982925298 cites W1979439941 @default.
• W1982925298 cites W1979801990 @default.
• W1982925298 cites W1985860736 @default.
• W1982925298 cites W2000719556 @default.
• W1982925298 cites W2002357201 @default.
• W1982925298 cites W2003991752 @default.
• W1982925298 cites W2018289835 @default.
• W1982925298 cites W2028507406 @default.
• W1982925298 cites W2044469448 @default.
• W1982925298 cites W2051432571 @default.
• W1982925298 cites W2052203027 @default.
• W1982925298 cites W2057700119 @default.
• W1982925298 cites W2065785294 @default.
• W1982925298 cites W2066441904 @default.
• W1982925298 cites W2067626667 @default.
• W1982925298 cites W2068733105 @default.
• W1982925298 cites W2070633083 @default.
• W1982925298 cites W2070693959 @default.
• W1982925298 cites W2074903810 @default.
• W1982925298 cites W2075536135 @default.
• W1982925298 cites W2075634016 @default.
• W1982925298 cites W2100837269 @default.
• W1982925298 cites W2101213713 @default.
• W1982925298 cites W2103151254 @default.
• W1982925298 cites W2115799430 @default.
• W1982925298 cites W2131695882 @default.
• W1982925298 cites W2142365365 @default.
• W1982925298 cites W2144206181 @default.
• W1982925298 cites W2149563853 @default.
• W1982925298 cites W40918469 @default.
• W1982925298 doi "https://doi.org/10.1093/nar/19.23.6595" @default.
• W1982925298 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/329226" @default.
• W1982925298 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1754396" @default.
• W1982925298 hasPublicationYear "1991" @default.
• W1982925298 type Work @default.
• W1982925298 sameAs 1982925298 @default.
• W1982925298 citedByCount "126" @default.
• W1982925298 countsByYear W19829252982012 @default.
• W1982925298 countsByYear W19829252982014 @default.
• W1982925298 countsByYear W19829252982015 @default.
• W1982925298 countsByYear W19829252982016 @default.
• W1982925298 countsByYear W19829252982018 @default.
• W1982925298 crossrefType "journal-article" @default.
• W1982925298 hasAuthorship W1982925298A5040793693 @default.
• W1982925298 hasAuthorship W1982925298A5080531304 @default.
• W1982925298 hasBestOaLocation W19829252981 @default.
• W1982925298 hasConcept C104317684 @default.
• W1982925298 hasConcept C121608353 @default.
• W1982925298 hasConcept C150903083 @default.
• W1982925298 hasConcept C153911025 @default.
• W1982925298 hasConcept C170493617 @default.
• W1982925298 hasConcept C202751555 @default.
• W1982925298 hasConcept C207001950 @default.
• W1982925298 hasConcept C2776067312 @default.
• W1982925298 hasConcept C2778938600 @default.
• W1982925298 hasConcept C51445715 @default.
• W1982925298 hasConcept C530470458 @default.
• W1982925298 hasConcept C54009773 @default.
• W1982925298 hasConcept C54355233 @default.
• W1982925298 hasConcept C552990157 @default.
• W1982925298 hasConcept C55493867 @default.
• W1982925298 hasConcept C84606932 @default.
• W1982925298 hasConcept C86803240 @default.
• W1982925298 hasConcept C96307122 @default.
• W1982925298 hasConceptScore W1982925298C104317684 @default.
• W1982925298 hasConceptScore W1982925298C121608353 @default.
• W1982925298 hasConceptScore W1982925298C150903083 @default.
• W1982925298 hasConceptScore W1982925298C153911025 @default.
• W1982925298 hasConceptScore W1982925298C170493617 @default.
• W1982925298 hasConceptScore W1982925298C202751555 @default.
• W1982925298 hasConceptScore W1982925298C207001950 @default.
• W1982925298 hasConceptScore W1982925298C2776067312 @default.
• W1982925298 hasConceptScore W1982925298C2778938600 @default.
• W1982925298 hasConceptScore W1982925298C51445715 @default.
• W1982925298 hasConceptScore W1982925298C530470458 @default.
• W1982925298 hasConceptScore W1982925298C54009773 @default.
• W1982925298 hasConceptScore W1982925298C54355233 @default.
• W1982925298 hasConceptScore W1982925298C552990157 @default.

• next