Matches in SemOpenAlex for { <https://semopenalex.org/work/W1983008595> ?p ?o ?g. }
- W1983008595 endingPage "95" @default.
- W1983008595 startingPage "85" @default.
- W1983008595 abstract "CRISPR (clustered regularly interspaced short palindromic repeat)/Cas (CRISPR-associated) is a nucleic acid processing system in bacteria and archaea that interacts with mobile genetic elements. CRISPR DNA and RNA sequences are processed by Cas proteins: in Escherichia coli K-12, one CRISPR locus links to eight cas genes (cas1, 2, 3 and casABCDE), whose protein products promote protection against phage. In the present paper, we report that purified E. coli Cas3 catalyses ATP-independent annealing of RNA with DNA forming R-loops, hybrids of RNA base-paired into duplex DNA. ATP abolishes Cas3 R-loop formation and instead powers Cas3 helicase unwinding of the invading RNA strand of a model R-loop substrate. R-loop formation by Cas3 requires magnesium as a co-factor and is inactivated by mutagenesis of a conserved amino acid motif. Cells expressing the mutant Cas3 protein are more sensitive to plaque formation by the phage λvir. A complex of CasABCDE ('Cascade') also promotes R-loop formation and we discuss possible overlapping roles of Cas3 and Cascade in E. coli, and the apparently antagonistic roles of Cas3 catalysing RNA-DNA annealing and ATP-dependent helicase unwinding." @default.
- W1983008595 created "2016-06-24" @default.
- W1983008595 creator A5000666204 @default.
- W1983008595 creator A5008740175 @default.
- W1983008595 creator A5020627322 @default.
- W1983008595 creator A5073044299 @default.
- W1983008595 date "2011-09-14" @default.
- W1983008595 modified "2023-10-06" @default.
- W1983008595 title "Helicase dissociation and annealing of RNA-DNA hybrids by <i>Escherichia coli</i> Cas3 protein" @default.
- W1983008595 cites W1573537615 @default.
- W1983008595 cites W1926535993 @default.
- W1983008595 cites W1966279389 @default.
- W1983008595 cites W1971115321 @default.
- W1983008595 cites W1971507610 @default.
- W1983008595 cites W1977531433 @default.
- W1983008595 cites W1977642897 @default.
- W1983008595 cites W1978094899 @default.
- W1983008595 cites W1978436270 @default.
- W1983008595 cites W1981844387 @default.
- W1983008595 cites W1983000410 @default.
- W1983008595 cites W1984592234 @default.
- W1983008595 cites W1989568784 @default.
- W1983008595 cites W1990570898 @default.
- W1983008595 cites W2007153971 @default.
- W1983008595 cites W2008790072 @default.
- W1983008595 cites W2010986636 @default.
- W1983008595 cites W2017608263 @default.
- W1983008595 cites W2022480099 @default.
- W1983008595 cites W2027591298 @default.
- W1983008595 cites W2029647341 @default.
- W1983008595 cites W2037913221 @default.
- W1983008595 cites W2048395044 @default.
- W1983008595 cites W2057092463 @default.
- W1983008595 cites W2057964816 @default.
- W1983008595 cites W2059781644 @default.
- W1983008595 cites W2062008243 @default.
- W1983008595 cites W2062280978 @default.
- W1983008595 cites W2067423587 @default.
- W1983008595 cites W2074136235 @default.
- W1983008595 cites W2074862753 @default.
- W1983008595 cites W2077190448 @default.
- W1983008595 cites W2088154229 @default.
- W1983008595 cites W2088502483 @default.
- W1983008595 cites W2093413052 @default.
- W1983008595 cites W2094722389 @default.
- W1983008595 cites W2097415632 @default.
- W1983008595 cites W2101788140 @default.
- W1983008595 cites W2101983500 @default.
- W1983008595 cites W2104549970 @default.
- W1983008595 cites W2118665281 @default.
- W1983008595 cites W2118746294 @default.
- W1983008595 cites W2133464522 @default.
- W1983008595 cites W2135077391 @default.
- W1983008595 cites W2139544568 @default.
- W1983008595 cites W2144566978 @default.
- W1983008595 cites W2144912899 @default.
- W1983008595 cites W2153537688 @default.
- W1983008595 cites W2153650494 @default.
- W1983008595 cites W2163241233 @default.
- W1983008595 cites W2163723712 @default.
- W1983008595 cites W2169244146 @default.
- W1983008595 cites W2170255645 @default.
- W1983008595 cites W2171152411 @default.
- W1983008595 doi "https://doi.org/10.1042/bj20110901" @default.
- W1983008595 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21699496" @default.
- W1983008595 hasPublicationYear "2011" @default.
- W1983008595 type Work @default.
- W1983008595 sameAs 1983008595 @default.
- W1983008595 citedByCount "59" @default.
- W1983008595 countsByYear W19830085952012 @default.
- W1983008595 countsByYear W19830085952013 @default.
- W1983008595 countsByYear W19830085952014 @default.
- W1983008595 countsByYear W19830085952015 @default.
- W1983008595 countsByYear W19830085952016 @default.
- W1983008595 countsByYear W19830085952017 @default.
- W1983008595 countsByYear W19830085952018 @default.
- W1983008595 countsByYear W19830085952019 @default.
- W1983008595 countsByYear W19830085952020 @default.
- W1983008595 countsByYear W19830085952021 @default.
- W1983008595 countsByYear W19830085952023 @default.
- W1983008595 crossrefType "journal-article" @default.
- W1983008595 hasAuthorship W1983008595A5000666204 @default.
- W1983008595 hasAuthorship W1983008595A5008740175 @default.
- W1983008595 hasAuthorship W1983008595A5020627322 @default.
- W1983008595 hasAuthorship W1983008595A5073044299 @default.
- W1983008595 hasConcept C104317684 @default.
- W1983008595 hasConcept C144501496 @default.
- W1983008595 hasConcept C153911025 @default.
- W1983008595 hasConcept C154055011 @default.
- W1983008595 hasConcept C161223559 @default.
- W1983008595 hasConcept C185581394 @default.
- W1983008595 hasConcept C54355233 @default.
- W1983008595 hasConcept C552990157 @default.
- W1983008595 hasConcept C67705224 @default.
- W1983008595 hasConcept C68592252 @default.
- W1983008595 hasConcept C86803240 @default.
- W1983008595 hasConcept C98108389 @default.
- W1983008595 hasConceptScore W1983008595C104317684 @default.