FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1983038576> ?p ?o ?g. }

• W1983038576 abstract "KLEIN, RICHARD M., EMERITA M. CAPUTO, and BARBARA A. WITTERHOLT. (New York Bot. Gard., N. Y., N. Y.) The role of zinc in the growth of plant tissue cultures. Amer. Jour. Bot. 49(4): 323-327. Illus. 1962.-Zinc-sufficient and zinc-deficient auxin-auxotrophic callus and auxin-prototrophic crown-gall cultures of Parthenocissus tricuspidatus were examined for their growth capacity and ability to synthesize tryptophan. Growth capacity decreased rapidly when zinc ion was withheld and tryptophan synthetase activity in vitro was reduced. Growth of -Zn crown-gall tissues was stimulated by either auxin or tryptophan and growth of -Zn callus cultures was stimulated by tryptophan. ZINC IONS are known to be required for a variety of metabolic processes in plants. The activities of a number of respiratory enzymes, the accumulation of quinones and catechol aggregates, respiratory impairment, changes in the levels of proteins and amino acids, and alteration in growth rates, have been reported to follow restrictions in the zinc supply (cf. McElroy and Nason, 1954). Of primary interest here are the reports on the role of zinc in auxin metabolism. Skoog (1940) reported that buds and stems of Zn tomato and sunflower plants contained virtually no free auxin. He postulated that zinc-deficient cells either inactivate auxins or that zinc may be required to maintain auxin in an active state. Tsui (1948) confirmed the findings of Skoog but concluded that zinc was necessary for the synthesis of tryptophan and hence, indirectly, for the synthesis of auxin. Harris (1954) also found that the auxin content of leaves and apical nodes of -Zn Gossypium was low, but noted that the auxin levels in -Zn stem and root tissues did not change appreciably. Tsui and Wu (1960) infiltrated leaves of tomato plants and found that tryptophan appeared to be synthesized only in leaves from plants having adequate levels of zinc. The necessity for zinc in tryptophan synthesis from serine and indole by Neurospora was demonstrated in vivo (Tatum and Bonner, 1944) and in vitro (Nason, 1950), and Gordon (1956) has summarized the evidence that tryptophan is a primary precursor of indole auxins in plants. Most recently, Yanofsky (1960) elucidated the pathways of tryptophan synthesis in microorganisms. The i-form of serine and either free indole or indole-3-glycerophosphate in the presence of an enzyme complex (tryptophan synthetase) forms free tryptophan. 1 Received for publication May 19, 1961. 2 Present address: Marymount-Manhattan College, New York, N. Y. Supported by grants from the National Science Foundation. The authors wish to thank Sister Helena Marie, O.P. (Aquinas High School, New York City) and Thomas Dunden, S.J. (St. Louis University) for their help. We are very grateful to Dr. Leonard Weinstein of the Boyce Thompson Institute for Plant Research for his kindness in performing the tryptophan analyses. Auxin-auxotrophic callus cultures and auxinprototrophic crown-gall cultures lend themselves admirably to a study of the role of zinc in auxin metabolism. Heller (1953) is, to our knowledge, the only worker who has conclusively demonstrated that tissue cultures deprived of zinc stop growing within 2 or 3 monthly transfers. Even one transfer to a Zn medium is followed by a drastic reduction in growth rate. The work reported at this time was designed to obtain information on the role of zinc in the auxincontrolled growth of tissue cultures. MATERIALS AND METHODS-Callus and crowngall tissue cultures of Parthenocissus tricuspidatus (Sieb. & Zucc.) Planch. were maintained on a modified White's agar medium in darkness at 25 C (Klein and Manos, 1960). For maintenance of the cultures, zinc ion at a final concentration of 0.1 mg/ml was supplied as the sulfate. The salts used for the nutrient solution were, with the exception of iron, analytical grade reagents. The FeCl3 used was the Speepure brand from Adam Hilger of London. The level of zinc ion in the Zn nutrient solution never exceeded 0.2 Ag/liter. All experimnents were conducted with tissues on 50 ml of the appropriate agar medium per 125-ml Erlenmeyer flask. A single piece of tissue was used per flask and 5-10 replicate cultures were used per variable. To eliminate the lag in growth which follows cutting of tissue cultures, the tissues were cut and transferred to media for 1 week before being weighed to the nearest mg on a Roller-Smith balance and transferred to experimental media. Tumor tissues averaged 200 mg and callus tissues 150 mg at this time. The cultures were incubated in darkness at 25 C for 30 days during which growth rates, as measured by fresh weight increments, were linear. Final fresh weight values were obtained to the nearest mg and growth was calculated as the percentage growth increment: Final fresh wt Initial fresh wt X 100" @default.
• W1983038576 created "2016-06-24" @default.
• W1983038576 creator A5028893279 @default.
• W1983038576 creator A5034054931 @default.
• W1983038576 creator A5081439041 @default.
• W1983038576 date "1962-04-01" @default.
• W1983038576 modified "2023-09-24" @default.
• W1983038576 title "THE ROLE OF ZINC IN THE GROWTH OF PLANT TISSUE CULTURES" @default.
• W1983038576 cites W1968632781 @default.
• W1983038576 cites W1990729744 @default.
• W1983038576 cites W2001653891 @default.
• W1983038576 cites W2002399290 @default.
• W1983038576 cites W2018039981 @default.
• W1983038576 cites W2035178202 @default.
• W1983038576 cites W2037025973 @default.
• W1983038576 cites W2206872516 @default.
• W1983038576 cites W4229686242 @default.
• W1983038576 cites W4231238649 @default.
• W1983038576 doi "https://doi.org/10.1002/j.1537-2197.1962.tb14945.x" @default.
• W1983038576 hasPublicationYear "1962" @default.
• W1983038576 type Work @default.
• W1983038576 sameAs 1983038576 @default.
• W1983038576 citedByCount "7" @default.
• W1983038576 countsByYear W19830385762012 @default.
• W1983038576 crossrefType "journal-article" @default.
• W1983038576 hasAuthorship W1983038576A5028893279 @default.
• W1983038576 hasAuthorship W1983038576A5034054931 @default.
• W1983038576 hasAuthorship W1983038576A5081439041 @default.
• W1983038576 hasConcept C104317684 @default.
• W1983038576 hasConcept C178790620 @default.
• W1983038576 hasConcept C185592680 @default.
• W1983038576 hasConcept C207778908 @default.
• W1983038576 hasConcept C2776706248 @default.
• W1983038576 hasConcept C2776928490 @default.
• W1983038576 hasConcept C515207424 @default.
• W1983038576 hasConcept C535196362 @default.
• W1983038576 hasConcept C55493867 @default.
• W1983038576 hasConcept C59822182 @default.
• W1983038576 hasConcept C86803240 @default.
• W1983038576 hasConcept C9549007 @default.
• W1983038576 hasConceptScore W1983038576C104317684 @default.
• W1983038576 hasConceptScore W1983038576C178790620 @default.
• W1983038576 hasConceptScore W1983038576C185592680 @default.
• W1983038576 hasConceptScore W1983038576C207778908 @default.
• W1983038576 hasConceptScore W1983038576C2776706248 @default.
• W1983038576 hasConceptScore W1983038576C2776928490 @default.
• W1983038576 hasConceptScore W1983038576C515207424 @default.
• W1983038576 hasConceptScore W1983038576C535196362 @default.
• W1983038576 hasConceptScore W1983038576C55493867 @default.
• W1983038576 hasConceptScore W1983038576C59822182 @default.
• W1983038576 hasConceptScore W1983038576C86803240 @default.
• W1983038576 hasConceptScore W1983038576C9549007 @default.
• W1983038576 hasFunder F4320306076 @default.
• W1983038576 hasLocation W19830385761 @default.
• W1983038576 hasOpenAccess W1983038576 @default.
• W1983038576 hasPrimaryLocation W19830385761 @default.
• W1983038576 hasRelatedWork W1970791420 @default.
• W1983038576 hasRelatedWork W2054995212 @default.
• W1983038576 hasRelatedWork W2086190015 @default.
• W1983038576 isParatext "false" @default.
• W1983038576 isRetracted "false" @default.
• W1983038576 magId "1983038576" @default.
• W1983038576 workType "article" @default.