Matches in SemOpenAlex for { <https://semopenalex.org/work/W1983059616> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W1983059616 endingPage "175" @default.
- W1983059616 startingPage "170" @default.
- W1983059616 abstract "The cDNA encoding a new type of alpha 2,3-sialyltransferase (mST3Gal V) was cloned from mouse brain cDNA library by PCR-based cloning approach using a pair of degenerate primers deduced from the nucleotide sequence information of mouse ST3Gal III and IV. The predicted amino acid sequence of mST3Gal V showed 27.3% and 26.4% identity to mST3Gal III and IV, respectively. The recombinant soluble mST3Gal V fused with protein-A, which expressed in the culture media of COS-7 cells, showed activity toward lactosylceramide (LacCer), and synthesized GM3. The apparent Km value for LacCer was 9.3 microM. mST3Gal V did not exhibit any activity toward other substrates we tested in this study, including glycolipids, glycoproteins and disaccharides. The mST3Gal V cDNA transfected F28-7 cells, which express large amount of lactosylceramide and very small amount of GM3 at native stage, expressed a large amount of GM3. The ST3Gal V gene was strongly expressed in mouse brain and liver, which contained a large amount of ganglioside. The gene expression seemed to be coincident with ganglioside expression in mouse. Thus, we conclude that mST3Gal V is the fifth-type alpha 2,3-sialyltransferase carrying GM3 synthetic activity." @default.
- W1983059616 created "2016-06-24" @default.
- W1983059616 creator A5003500065 @default.
- W1983059616 creator A5005960995 @default.
- W1983059616 creator A5017249338 @default.
- W1983059616 creator A5030613587 @default.
- W1983059616 creator A5047201524 @default.
- W1983059616 creator A5057287134 @default.
- W1983059616 creator A5060389174 @default.
- W1983059616 creator A5083253988 @default.
- W1983059616 date "1998-12-01" @default.
- W1983059616 modified "2023-10-10" @default.
- W1983059616 title "Molecular Cloning and Functional Expression of a Fifth-Type α2,3-sialyltransferase (mST3Gal V: GM3 synthase)" @default.
- W1983059616 doi "https://doi.org/10.1006/bbrc.1998.9768" @default.
- W1983059616 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9875239" @default.
- W1983059616 hasPublicationYear "1998" @default.
- W1983059616 type Work @default.
- W1983059616 sameAs 1983059616 @default.
- W1983059616 citedByCount "63" @default.
- W1983059616 countsByYear W19830596162012 @default.
- W1983059616 countsByYear W19830596162014 @default.
- W1983059616 countsByYear W19830596162015 @default.
- W1983059616 countsByYear W19830596162016 @default.
- W1983059616 countsByYear W19830596162017 @default.
- W1983059616 countsByYear W19830596162018 @default.
- W1983059616 countsByYear W19830596162019 @default.
- W1983059616 countsByYear W19830596162020 @default.
- W1983059616 countsByYear W19830596162021 @default.
- W1983059616 crossrefType "journal-article" @default.
- W1983059616 hasAuthorship W1983059616A5003500065 @default.
- W1983059616 hasAuthorship W1983059616A5005960995 @default.
- W1983059616 hasAuthorship W1983059616A5017249338 @default.
- W1983059616 hasAuthorship W1983059616A5030613587 @default.
- W1983059616 hasAuthorship W1983059616A5047201524 @default.
- W1983059616 hasAuthorship W1983059616A5057287134 @default.
- W1983059616 hasAuthorship W1983059616A5060389174 @default.
- W1983059616 hasAuthorship W1983059616A5083253988 @default.
- W1983059616 hasConcept C104317684 @default.
- W1983059616 hasConcept C108625454 @default.
- W1983059616 hasConcept C121050878 @default.
- W1983059616 hasConcept C153911025 @default.
- W1983059616 hasConcept C187882448 @default.
- W1983059616 hasConcept C19924922 @default.
- W1983059616 hasConcept C199360897 @default.
- W1983059616 hasConcept C22222904 @default.
- W1983059616 hasConcept C2777331542 @default.
- W1983059616 hasConcept C2778322149 @default.
- W1983059616 hasConcept C2910328161 @default.
- W1983059616 hasConcept C41008148 @default.
- W1983059616 hasConcept C55493867 @default.
- W1983059616 hasConcept C86803240 @default.
- W1983059616 hasConceptScore W1983059616C104317684 @default.
- W1983059616 hasConceptScore W1983059616C108625454 @default.
- W1983059616 hasConceptScore W1983059616C121050878 @default.
- W1983059616 hasConceptScore W1983059616C153911025 @default.
- W1983059616 hasConceptScore W1983059616C187882448 @default.
- W1983059616 hasConceptScore W1983059616C19924922 @default.
- W1983059616 hasConceptScore W1983059616C199360897 @default.
- W1983059616 hasConceptScore W1983059616C22222904 @default.
- W1983059616 hasConceptScore W1983059616C2777331542 @default.
- W1983059616 hasConceptScore W1983059616C2778322149 @default.
- W1983059616 hasConceptScore W1983059616C2910328161 @default.
- W1983059616 hasConceptScore W1983059616C41008148 @default.
- W1983059616 hasConceptScore W1983059616C55493867 @default.
- W1983059616 hasConceptScore W1983059616C86803240 @default.
- W1983059616 hasIssue "1" @default.
- W1983059616 hasLocation W19830596161 @default.
- W1983059616 hasLocation W19830596162 @default.
- W1983059616 hasOpenAccess W1983059616 @default.
- W1983059616 hasPrimaryLocation W19830596161 @default.
- W1983059616 hasRelatedWork W1957304069 @default.
- W1983059616 hasRelatedWork W1984886787 @default.
- W1983059616 hasRelatedWork W2020075783 @default.
- W1983059616 hasRelatedWork W2021471996 @default.
- W1983059616 hasRelatedWork W2045706861 @default.
- W1983059616 hasRelatedWork W2079306323 @default.
- W1983059616 hasRelatedWork W2087942040 @default.
- W1983059616 hasRelatedWork W2154409260 @default.
- W1983059616 hasRelatedWork W2802179000 @default.
- W1983059616 hasRelatedWork W4313446186 @default.
- W1983059616 hasVolume "253" @default.
- W1983059616 isParatext "false" @default.
- W1983059616 isRetracted "false" @default.
- W1983059616 magId "1983059616" @default.
- W1983059616 workType "article" @default.